Ultraviolet Irradiation of Bacillus thuringiensis Bt64 and Its Pathogenicity to Naranga aenescens

doi:10.16409/j.cnki.2095-039x.2024.03.006

Abstract

Abstract: To explore the ultraviolet resistance and the pathogenicity changes on Naranga aenescens of Bacillus thuringiensis Bt64 induced by ultraviolet irradiation, this study revealed the effects of different ultraviolet irradiation treatments on the growth and development of B. thuringiensis Bt64 and the change in UV resistance. The toxicity changes and outdoor control effects of Bt64 strain on N. aenescens larvae after ultraviolet treatment were also clarified. The results showed that the colony forming units and spore yield of continuous UV irradiation at different times were all higher than those of direct UV irradiation. The inhibition rate of spore germination was lower than that of direct UV irradiation in different time, and the difference was significant with the increase of irradiation duration. Under the conditions of 30 minutes and 45 minutes irradiation, the pathogenicity and control effect of the rejuvenated strain after continuous UV irradiation and the rejuvenated strain were irradiated again after continuous UV irradiation on the N. aenescens larvae were enhanced, which was significantly higher than that of direct UV irradiation treatment at the same time. The result shows that UV resistant mutant can be selected by continuous UV irradiation, which can enhance the UV resistance, insecticidal activity and outdoor control effect of Bt64 strain, and provide a basis for the development of new biopesticides with high insecticidal activity and persistence.

Key words: Bacillus thuringiensis, ultraviolet, Naranga aenescens, strain growth and development, pathogenicity

CLC Number:

S476.1

MA Shiyan, HAN Yutong, LIU Xiaoli, ZHANG Xinxin, YU Hongchun. Ultraviolet Irradiation of Bacillus thuringiensis Bt64 and Its Pathogenicity to Naranga aenescens[J]. Chinese Journal of Biological Control, 2024, 40(6): 1275-1284.

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