Biocontrol Efficiency of Trichoderma longibrachiatum Wettable Powder on Atractylodes chinensis Seeding Blight Caused by Rhizoctonia solani

doi:10.16409/j.cnki.2095-039x.2025.02.082

Abstract

Abstract: To evaluate the control efficacy of Trichoderma wettable powder (WP) against seedling blight, an important disease caused Atractylodes chinensis seedling death, and its growth-promoting effects on A. chinensis under different application methods, TR₉ strain of Trichoderma longibrachiatum was screened using dual culture and plate-on-plate assays. The control efficacy of different application methods of Trichoderma WP on seedling blight and its growth-promoting effects on A. chinensis seedlings were tested in the field. The results showed that inhibition rate of T. longibrachiatum TR₉ and its volatile metabolites against R. solani were 82.39% and 70.98%, respectively. Greenhouse experiments indicated that seed dressing with TR₉ WP 1:30 and seed quality ratio resulted in a control efficacy of 87.33% against A. chinensis seedling blight, with a seedling survival rate of 92.67%. Among the surviving seedlings， the rhizome diameters of grade Ⅰ and grade Ⅱ seedlings were also the highest in the 1:30 of TR₉ WP seed dressing treatment, reaching 0.780 cm and 0.543 cm, respectively. Additionally, TR₉ WP significantly increased the leaf area and chlorophyll a content of A. chinensis to 10.61 cm² and 1.811 mg/g, respectively, which were significantly higher than those in the carbendazim WP seed dressing control and the water control. Field trials demonstrated that seed dressing with 1:30 of TR₉ WP, followed by root irrigation with TR₉ WP 200-fold dilution at the seedling stage, achieved a control efficacy of 83.07% against A. chinensis seedling blight, showing no significant difference from the control efficacy of 83.61% achieved by root irrigation with metalaxyl·fludioxonil. It can be concluded that Trichoderma longibrachiatum TR₉ WP can be used to control A. chinensis seedling blight.

Key words: Trichoderma longibrachiatum, wetting powder, Atractylodes chinensi seeding blight, control efficiency

CLC Number:

A476+.12

ZHANG Yuping, LI Yuqi, GAO Yufeng, LU Guoyan, FAN Hairong, YANG Congzhou, HE Zidian, CHEN Jie. Biocontrol Efficiency of Trichoderma longibrachiatum Wettable Powder on Atractylodes chinensis Seeding Blight Caused by Rhizoctonia solani[J]. Chinese Journal of Biological Control, 2025, 41(6): 1421-1429.

References

[1] 曹春梅, 王晓娇, 许飞, 等. 内蒙古地区马铃薯黑痣病立枯丝核菌融合群及致病性研究[J]. 中国马铃薯, 2018, 32(5): 293-302.
[2] 黄江华, 杨媚, 周而勋, 等. 13种植物丝核菌对水稻、甜玉米、黄瓜和甘蓝的交互致病性[J]. 华中农业大学学报, 2008(2): 198-203.
[3] 蓝达愉. 广西烟草叶部病害的病原菌鉴定及快速检测[D]. 南宁: 广西大学, 2023.
[4] 王朵. 十字花科蔬菜丝核菌根腐病的病原生物学及检测技术研究[D]. 北京: 中国农业科学院, 2020.
[5] 智慧. 白薇病害鉴定及丝核菌叶斑病病原学研究[D]. 沈阳: 沈阳农业大学, 2021.
[6] 谢昀烨. 吉林省主要药用植物丝核菌属真菌病害研究[D]. 长春: 吉林农业大学, 2013.
[7] 鹿秀云, 商俊燕, 苏振贺, 等. 北沙参根腐病病原鉴定及侵染特点分析[J]. 植物病理学报, 2023, 53(5): 959-965.
[8] 王树桐. 板蓝根根腐病病原学及生物防治研究[D]. 保定: 河北农业大学, 2012.
[9] 冉晓敏, 金榕榕, 易春梅, 等. 立枯丝核菌的防控研究[J]. 农业灾害研究, 2018, 8(5): 4-5, 11.
[10] 张娜, 梁巧兰, 吴琼. 花椰菜苗期立枯病病原鉴定及室内药剂筛选[J]. 甘肃农业大学学报, 2018, 53(1): 65-70.
[11] 向立刚, 汪汉成, 蔡刘体, 等. 五种杀菌剂对烟草立枯病菌的生物活性(英文)[J]. 农药学学报, 2023, 25(1): 81-88.
[12] 黄艺烁, 谢学文, 石延霞, 等. 多粘类芽胞杆菌ZF197对白菜茎基腐病防治效果[J]. 园艺学报, 2020, 47(6): 1059-1071.
[13] 罗文芳, 周军辉, 何伟, 等. 萎缩芽胞杆菌LB6-2抑制立枯丝核菌活性物质的鉴定[J]. 中国生物防治学报, 2024, 40(2): 378-387.
[14] 张丽芳, 曹阳, 张兰. 哈茨木霉T-102菌剂的制备及其对花生纹枯病和土壤生物多样性的影响[J]. 中国油料作物学报, 2024, 46(6): 1398-1404.
[15] 李雯, 王天君, 台莲梅, 等. 拟康宁木霉78对马铃薯黑痣病防效及土壤酶活性的影响[J]. 江苏农业科学, 2024, 52(3): 159-163.
[16] 方中达. 植病研究方法[M]. 北京: 中国农业出版社, 1998.
[17] 孙丽丽, 曹传旺, 薛绪亭, 等. 棘孢木霉可湿性粉剂研制及杀菌活性测定[J]. 北京林业大学学报, 2015, 7(6): 45-52.
[18] 中华人民共和国国家质量监督检验检疫总局. 农药可湿性粉剂润湿性测定方法:GB/T 5451-2001[S]. 北京: 中国标准出版社, 2001.
[19] 中华人民共和国国家质量监督检验检疫总局. 农药悬浮率测定方法:GB/T14825-2006[S]. 北京: 中国标准出版社, 2007.
[20] 中华人民共和国国家质量监督检验检疫总局. GB/T25864-2010球孢白僵菌粉剂[S]. 北京: 中国标准出版社, 2011.
[21] 中华人民共和国国家质量监督检验检疫总局. GB/T 1600-3 pH值测定方法[S]. 北京: 中国标准出版社, 1993.
[22] 李志丹, 韩瑞宏, 廖桂兰, 等. 植物叶片中叶绿素提取方法的比较研究[J]. 广东第二师范学院学报, 2011, 31(3): 81-83.
[23] 陈碧云, 周乐聪, 陆致平. 绿色木霉发酵配方与防治油菜菌核病的研究[J]. 中国生物防治学报, 2001, 17(2): 67-70.
[24] Dou J, Liu J, Ma G, et al. The physiological effect of Trichoderma viride on melon yield and its ability to suppress Rhizoctonia solani[J]. Agronomy, 2024, 14(10): 2318.
[25] Sawant S S, Bhapkar R S, Choi E, et al. Potential of Trichoderma species to control Rosellinia necatrix,the etiological agent of white root rot[J]. Biological Control, 2024, 199: 10566.
[26] 张晓尘, 张红杰, 李生兵, 等. 哈茨木霉EMF910对宁夏盐碱地区黄芪根腐病致病菌的防治作用[J]. 微生物学通报, 2024, 51(10): 4162-4180.
[27] 贺字典, 韩亚梅, 金歌, 等. 木霉菌协同杀菌剂防治北苍术白绢病的增效作用[J]. 中国生物防治学报, 2023, 39(5): 1244-1252.
[28] Mukhopadhyay A, Mukherjee S, Dutta S, et al. Evaluating the bioactivity of Trichoderma asperellum against Colletotrichum siamense and its growth-promoting effects on Aloe vera (Aloe barbadensis Mill.)[J]. European Journal of Plant Pathology, 2025, 172(2): 275-289.
[29] Özkale E, Yörük E, Budak M, et al. Trichoderma atroviride suppresses Fusarium graminearum by altering primary and secondary metabolite biosynthesis profiling[J]. Plant Pathology, 2023, 72(8): 1428.
[30] 付宏喆, 杨盟权, 彭智良, 等. 烟草根腐病生防木霉菌筛选及防病作用研究[J]. 中国烟草学报, 2025, 31(2): 122-131.
[31] 景芳, 张树武, 刘佳, 等. 长枝木霉T6生防菌剂发酵条件优化及其对辣椒立枯病的防治效果[J]. 中国生物防治学报, 2020, 36(1): 113-124.
[32] 李心芃, 宋瑞轲, 高琦, 等. 哈茨木霉菌活性物质Harzianic Acid的异源合成及其抑菌机制研究[J]. 南京农业大学学报, 2025, 48(4): 880-891.
[33] 曹秋林, 李菁菁, 廉华, 等. 防治花生白绢病木霉菌的筛选及生防特性研究[J]. 中国油料作物学报, 2025, 47(5): 1189-1198.
[34] Alwadai S A, Wahibi A S M, Alsayed F M, et al. Molecular characterization of plant growth-promoting Trichoderma from Saudi Arabia[J]. Scientific Reports, 2024, 14(1): 1-20.
[35] Shamla A K, Chitra A V. Assessing the impact of Terminalia arjuna plant extracts on the growth and sporulation of Trichoderma harzianum,Aspergillus niger and Penicillium chrysogenum[J]. Applied and Environmental Microbiology, 2024, 18(4): 2592-2605.
[36] 赵青松, 吴凡, 贺莹莹, 等. 中药渣堆肥中拮抗真菌的筛选、鉴定[J]. 北方园艺, 2022(3): 86-91.
[37] 李泽方, 罗志会, 刘慕兰, 等. 膏状剂型的木霉菌生物农药组合物及其制备方法[P].中国发明专利, CN200910264403.6, 2010-11-3.
[38] 于稳欠, 陈晨, 糜芳, 等. 一种木霉菌生物农药泡腾片剂及其制备方法[P]. 中国发明专利, CN201910848533.8, 2019-11-29.
[39] 孙丽丽, 曹传旺, 薛绪亭, 等. 棘孢木霉可湿性粉剂研制及杀菌活性测定[J]. 北京林业大学学报, 2015, 37(6): 45-52.
[40] 陈路生, 吴晓儒, 白真旭, 等. 木霉复合颗粒剂创制技术与应用[J]. 中国生物防治学报, 2024, 40(4): 874-883.
[41] 牛鑫斌, 徐秉良, 刘佳, 等. 长枝木霉T6水分散粒剂对3 种植物病害的生防效果[J]. 甘肃农业大学学报, 2018, 53(1): 90-94,101.
[42] 戴启星. 长枝木霉T6菌株可湿性粉剂研制及复配菌剂协同增效作用研究[D]. 兰州: 甘肃农业大学, 2019.
[43] Morcuende J, García M J, Velasco P, et al. Effective biological control of chickpea rabies (Ascochyta rabiei) through systemic phytochemical defenses activation by Trichoderma roots colonization: from strain characterization to seed coating[J]. Biological Control, 2024, 193: 105530.
[44] Wang Y P, Wang J, Zhu X C, et al. Genome and transcriptome sequencing of Trichoderma harzianum T4, an important biocontrol fungus of Rhizoctonia solani, reveals genes related to mycoparasitism[J]. Canadian Journal of Microbiology, 2024, 70: 86-101.