Control Effects and Related Biocontrol Factors Detection of Two Bacillus amyloliquefaciens Strains on Watermelon Fusarium Wilt

doi:10.16409/j.cnki.2095-039x.2018.05.012

Abstract

Abstract: Control effects of two Bacillus strains TR2 and CE on watermelon Fusarium wilt were investigated and their related biocontrol factors were detected. Plate confrontation tests showed that the two Bacillus strains had growth inhibitory effects on watermelon Fusarium wilt strain Xg-1, Xg-2 and Xg-3. Strain TR2 showed the best inhibitory effect on Xg-1with the width of inhibition zone of 4.7 mm. Strain CE showed the best inhibitory effect on Xg-3 with the width of inhibition zone of 5.0 mm. Mycelial deformation such as being thinner, distorted, and some cells expanded into spherical shapes could be observed along the edge of inhibition zones. Watermelon seedling inoculation test showed that watermelon Fusarium wilt could be controlled by strains TR2 and CE. The control effect of TR2 on pathogen Xg-1 reached to 60% with the disease index decreased significantly on the fifteenth day. On the third day, CE had the best control efficacy of 63.6%. Related biocontrol factors of TR2 and CE were detected by crystal violet staining, molybdenum antimony colorimetric, 4 benzene boron sodium assay and transparent circle detection. Both TR2 and CE had the ability of forming biofilm, decomposing of organic phosphorus, inorganic phosphorus and potassium ore, producing protease, amylase and cellulase. Tested Hydroponic culture experiment demonstrated that TR2 and CE could promote the root and stem growth of watermelon seedlings due to their phosphate and potassium solubilizing capacity. The above results indicated that TR2 and CE could control watermelon Fusarium wilt disease, form biofilm and colonize around plant root, and promote the growth of watermelon seedlings, which have the potential to be developed as biocontrol agents.

Key words: biocontrol Bacillus, watermelon Fusarium wilt, inhibition activity, control effect, biocontrol factors

CLC Number:

S476

LI Dan, LI Rongmei, QIN Weiying, ZHOU Yu, SHANG Qiaoxia, REN Zhengguang, WEI Yanmin, ZHAO Xiaoyan. Control Effects and Related Biocontrol Factors Detection of Two Bacillus amyloliquefaciens Strains on Watermelon Fusarium Wilt[J]. journal1, 2018, 34(5): 729-737.

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