Synergistic Effect of Trichoderma and Fungicides on Atractylodes chinensis Southern Blight Caused by Sclerotium rolfsii

doi:10.16409/j.cnki.2095-039x.2023.02.046

Abstract

Abstract: In order to control effectively southern blight caused by Sclerotium rolfsii of Atractylodes chinensis as well as reducing the dosage of chemical fungicides, Trichoderma strains that can be used in combination with fungicides were screened out. Toxicity of 12 chemical fungicides agents and 7 Trichoderma stains against S. rolfsii was firstly tested in vitro with toxic medium method. And Trichoderma asperellum T₁₆, Trichoderma atroviride T₁₅ and Trichoderma harzianum T₁₁ were subsequently shown to exhibit bio-compatibility with fungicides proved by dural culture and synergistic effect method. Control efficacy of the combination on suppressing of southern blight of A.chinensis was further tested in field experiment. The results showed that the VEC₅₀between240 g/L thifluzamide SC, 30% hymexazol AS and 80% mancozeb WP with T. asperellum T₁₆, T. atroviride T₁₅ and T. harzianum T₁₁ were higher than 1, respectively, which indicated good bio-compatibility. In the reducing half dosage of thifluzamide condition, the synergistic control effect of thifluzamide combined with T. asperellum T₁₆ and T. atroviride T₁₅ on southern blight reached 80.76% and 78.82%, respectively, which increased by 13.36% and 10.64% compared with thifluzamide control. In conclusion, T. asperellum T₁₆ and T. atroviride T₁₅could synergize with thifluzamide to control A. chinensis southern blight, which was much more effective than applying 100% fungicides.

Key words: Atractylodes chinensis southern blight disease, Trichoderma asperellum, Trichoderma atroviride, thifluzamide, synergistic effect

CLC Number:

S476

HE Zidian, HAN Yamei, JIN Ge, GAO Yufeng, MIN Kang. Synergistic Effect of Trichoderma and Fungicides on Atractylodes chinensis Southern Blight Caused by Sclerotium rolfsii[J]. Chinese Journal of Biological Control, 2023, 39(5): 1244-1252.

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