Differentiation and Expression Analysis of HGO Genes among Different Haplotypes of Cotton Aphids

doi:10.16409/j.cnki.2095-039x.2023.11.003

Abstract

Abstract: Cotton aphid, Aphis gossypii Glover, differentiated into biotypes, showing obvious differences in host preference among the biotypes and differentiation at the genome level. In order to understand the evolution of hormone synthesis-related genes during the biotype differentiation of cotton aphids, farnesol dehydrogenase (HGO) of Hap1 and Hap3 cotton aphids was selected for analysis. According to the genome information of the two biotypes released by our laboratory, five AgosHGO genes were identified in each of the genomes of Hap1 and Hap3 cotton aphids, four of which are repetitive copies locate on chromosome A1 and the other one locates on chromosome A2. The position of the HGO gene on the chromosome, gene coding and sequence composition were analyzed to obtain relevant evolution information. The SRA data of cotton aphid published by NCBI were used to analyze the expression changes of HGO gene under different treatment conditions. There were differences in CDS sequence and gene structure of AgosHGO among different biotypes. In the Hap3 cotton aphid, a Gypsy transposon insertion is in the region of AgosHGO gene which distributes on chromosome A1.The analysis showed that the expression of AgosHGO was significantly different among different hosts but did not change with body color and temperature. The expression of AgosHGO3 was significantly down-regulated by 50% after acetamiprid treatment. The expression of AgosHGO5 in male aphid was significantly up-regulated in adult instar compared with the 1^st—4th instar, up to 14—20 times. This study confirms that the HGO gene differentiates in Hap1 and Hap3 cotton aphids, its expression level can respond to its own development and changes in the external environment. It is of great significance to develop biological control technologies based on the regulation of hormone.

Key words: Aphis gossypii, population differentiation, farnesol dehydrogenase, genome, sequence structure

CLC Number:

S476

YANG Xinyi, ZHANG Yaling, CAO Xue, JI Jing, ZHANG Shuai. Differentiation and Expression Analysis of HGO Genes among Different Haplotypes of Cotton Aphids[J]. Chinese Journal of Biological Control, 2024, 40(4): 844-855.

References

[1] 张帅, 吕丽敏, 王春义, 等. 高通量测序技术研究棉蚜携带病毒种类[J]. 棉花学报, 2014, 26(6): 539-545.
[2] 晁文娣, 吕昭智, 赵莉, 等. 七星瓢虫对不同初始密度棉蚜种群的调控作用[J]. 环境昆虫学报, 2021, 43(1): 206-213.
[3] 刘向东, 翟保平, 张孝羲, 等. 棉花型和瓜型棉蚜形态和生态适应力的分化[J]. 昆虫学报, 2004, 47(6): 6.
[4] 高雪, 刘向东. 棉花型和瓜型棉蚜产生有性世代能力的分化[J]. 昆虫学报, 2008, 51(1): 6.
[5] Zhang S, Luo J Y, Wang L, et al. The biotypes and host shifts of cotton-melon aphids Aphis gossypii in northern China[J]. Journal of Integrative Agriculture, 2018, 17(9): 2066-2073.
[6] 张思宇. 激素调控下荻草谷网蚜翅型分化的分子机制解析[D]. 武汉: 长江大学, 2022.
[7] Ishikawa A, Gotoh H, Abe T, et al. Juvenile hormone titer and wing-morph differentiation in the vetch aphid Megoura crassicauda[J]. Journal of Insect Physiology, 2013, 59(4): 444-449.
[8] Riddiford L M. How does juvenile hormone control insect metamorphosis and reproduction?[J] General & Comparative Endocrinology, 2012, 179(3): 477-484.
[9] Zou Z, Saha T T, Roy S, et al. Juvenile hormone and its receptor, methoprene-tolerant, control the dynamics of mosquito gene expression[J]. Proceedings of the National Academy of Ences, 2013, 110(24): E2173-E2181.
[10] 孙亚亚. 华山松大小蠹保幼激素合成与信号传导基因功能研究[D]. 阳凌: 西北农林科技大学, 2022.
[11] Kumar R, Das J, Mahto J K, et al. Crystal structure and molecular characterization of NADP plus-farnesol dehydrogenase from cotton bollworm, Helicoverpa armigera[J]. Insect Biochemistry and Molecular Biology, 2022, 147: 103812.
[12] Mayoral J G, Nouzova M, Navare A, et al. NADP(+)-dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(50): 21091-21096.
[13] Zifruddin A N, Mohamad-Khalid K A, Suhaimi S A, et al. Molecular characterization and enzyme inhibition studies of NADP(+)-farnesol dehydrogenase from diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)[J]. Bioscience Biotechnology and Biochemistry, 2021, 85(7): 1628-1638.
[14] Satyaveanthan M V, Ng C L, Awang A, et al. Isolation, purification and biochemical characterization of Conopomorpha cramerella farnesol dehydrogenase[J]. Insect Molecular Biology, 2023, 32(2): 143-159.
[15] 宋中豪. MiR-318参与桔小实蝇卵巢发育的分子机制探究[D]. 重庆: 西南大学, 2021.
[16] Mao L, Henderson G. Group size effect on worker juvenile hormone titers and soldier differentiation in Formosan subterranean termite[J]. Journal of Insect Physiology, 2010, 56(7): 725-730.
[17] 楚君鹏. 保幼激素通路相关基因参与白蚁兵蚁分化的研究[D]. 郑州: 河南农业大学, 2019.
[18] Zhang S, Gao X, Wang L, et al. Chromosome-level genome assemblies of two cotton-melon aphid Aphis gossypii biotypes unveil mechanisms of host adaption[J]. Molecular Ecology Resources, 2022, 22(3): 1120-1134.
[19] 张广学, 赵季秋, 朱淑范, 等. 利用当地天敌综合治理棉蚜的试验研究[J]. 植物保护学报, 1982(2): 89-94.
[20] 孟玲, 李保平. 新疆棉蚜生物型的研究[J]. 棉花学报, 2001(1): 30-35.
[21] 伍一军. 近二十年我国杀虫剂毒理学研究进展(Ⅱ)——昆虫对杀虫剂的抗性研究[J]. 应用昆虫学报, 2020, 57(5): 995-1008.
[22] 梁彦, 张帅, 邵振润, 等. 棉蚜抗药性及其化学防治[J]. 植物保护, 2013, 39(5): 70-80.
[23] 张帅, 李超侠, 康颖, 等. 细胞色素P450基因在棉蚜生物型间的分化[J]. 植物保护, 2023, 49(1): 178-186, 199.
[24] 张泽麟, 嵇杰, 徐文雨, 等. MiR-252-5p通过靶向调控保幼激素酸甲基转移酶基因JHAMT的表达而影响果蝇变态发育[J]. 昆虫学报, 2022, 65(7): 799-806.
[25] 赵娜, 叶小芳, 程文玉, 等. 意大利蝗卵发育过程保幼激素和蜕皮激素含量的变化及其代谢相关基因的表达谱[J]. 昆虫学报, 2023, 66(2): 121-129.
[26] 刘素宁, 任充华, 李胜. 昆虫变态发育的激素和营养调控研究进展与展望[J]. 应用昆虫学报, 2019, 56(4): 13.
[27] Yin Y, Qiu Y W, Huang J, et al. Enzymes in the juvenile hormone biosynthetic pathway can be potential targets for pest control[J]. Pest Management Science, 2020, 76(3): 1071-1077.
[28] 韩光杰. 昆虫SINE和Helitron转座子的鉴定、进化及在二化螟中的胁迫响应[D]. 扬州: 扬州大学, 2021.