Optimisation of Conditions for the Proliferation of DpCPV by Alternative Host, Spodoptera exigua (Lepidoptera: Noctuidae)

doi:10.16409/j.cnki.2095-039x.2024.11.009

Abstract

Abstract: Spodoptera exigua is one of the dominant alternative hosts for the proliferation of Dendrolimus punctatus cytoplasmic polyhedrosis virus (DpCPV). The optimal conditions for the proliferation of DpCPV in S. exigua were determined in this study through employing an in vivo amplification method to compare the effects of various DpCPV inoculum doses, larval stage, inoculation frequency, feeding temperature and larval density on proliferation of DpCPV. The results showed that under the same DpCPV inoculum doses, the proliferation of DpCPV in S. exigua increased first and then decreased. With the increase of DpCPV inoculum doses, the proliferation of DpCPV in the 2^nd instar larvae of S. exigua increased first and then decreased, and the proliferation of DpCPV in the 3^rd to 5th instar larvae of S. exigua continued to increase. In addition, the proliferation of DpCPV decreased with the increase of inoculation frequency, feeding temperature and larval density. These results showed that the optimal combination conditions for the proliferation of DpCPV in S. exigua are: DpCPV inoculum concentration of 1×10⁶ PIB/mL (the inoculum dose of 5×10³ PIB/larva) in the 3^rd instar larvae of S. exigua, and the inoculation frequency of 1 time, the feeding temperature of 20 ℃, the feeding density of 100 larvae/box (0.05 larvae/cm²), which resulted in proliferation of DpCPV at 2.01×10⁹ PIB/larva. The results provide theoretical basis and technical support for industrialized production and forest application of DpCPV.

Key words: alternative host, Spodoptera exigua, Dendrolimus punctatus cytoplasmic polyhedrosis virus (DpCPV), virus proliferation, condition optimization

CLC Number:

S476.1

ZHAO Zhengping, ZHANG Min, XIA Yonggang, ZHONG Wuhong, YAN Xuewu. Optimisation of Conditions for the Proliferation of DpCPV by Alternative Host, Spodoptera exigua (Lepidoptera: Noctuidae)[J]. Chinese Journal of Biological Control, 2025, 41(4): 819-825.

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