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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (3): 662-672.DOI: 10.16409/j.cnki.2095-039x.2022.02.020

• RESEARCH REPORTS • Previous Articles    

Screening, Identification and Biological Control Activity of Streptomyces sp. XG40 against Black Spot Disease of Guanxi Honey Pomelo Cultivar

LAI Baochun1, DAI Ruiqing1, WU Zhenqiang1, YAO Jinai2, ZENG Tianbao1, WANG Jiarui1   

  1. 1. Zhangzhou Institute of Agricultural Sciences of Fujian Province, Zhangzhou 363005, China;
    2. Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
  • Received:2021-10-13 Published:2022-06-20

Abstract: To screen the strains with antagonistic effect against Phyllosticta citriasiana, the plant rhizosphere actinomyces strains were isolated and screened by plate-confrontation and oxford cup method. A total of 12 actinomyces strains had inhibition activity against P. citriasiana, and the actinomyces XG40 was the strongest, with inhibition zone diameter reaching to 16.3 mm. Based on the morphological, physiobiochemical characteristics and 16S rDNA、recArpoBgyrB sequence analysis, XG40 was identified as Streptomyces catenulae. The antifungal spectrum experiment indicated that Streptomyces catenulae XG40 also showed broad-spectrum inhibition activity to 12 plant pathogenic fungi and 2 plant pathogenic bacteria, such as Fulvia fulva, Colletotrichum gloeosporioides and Diaporthe citri. The inhibition mechanism of XG40 against P. citriasiana was investigated by optical Microscope observation showed that the hyphae inhibited by the strain XG40 was distorted, with uneven thickness, and broken in large quantity. Fermentative liquid of XG40 could dissolve conidia, and produce vacuoles simultaneously. In addition, the prevention and cure efficacies of the fermented broth against P. citriasiana were up to 83.52% and 78.87% in vitro, 78.91% and 71.15% in field, respectively. Therefore, strain XG40 could be used for controlling the honey pomelo black spot disease, and has a wonderful potential for application.

Key words: Streptomyces, antimicrobial activity, identification, honey pomelo black spot disease, biocontrol efficacy

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