Effects of GLUT Gene Expression on Energy Metabolism in Harmonia axyridis

doi:10.16409/j.cnki.2095-039x.2022.03.013

Abstract

Abstract: Harmonia axyridis is an important predatory ladybug that preys on a variety of aphids and other pests. At present, it has been widely used in the biological control of agricultural pests all over the world. In order to achieve rapid propagation of ladybugs at low cost in a short period of time, a lot of research has been done on the formula of artificial diet for ladybugs, so that they can complete growth and development. The artificial diet supplemented with trehalose and glucose was screened out in the laboratory, which can increase ladybug larval body weight, shorten the pre-oviposition period, and increase fecundity and the egg hatching rate, and benefit the growth, development and reproduction of the ladybugs. With ladybugs feeding aphid as the control group, the expression of two glucose transporter genes (GLUT1 and GLUT4) at various developmental stages of H. axyridis was analyzed, and the RNA interference technology was used to further explore the expression of dsGLUT1 or dsGLUT4 after injection. The expression and enzymatic activity of energy metabolism-related genes in H. axyridis proved that this gene is closely related to the regulation function of energy metabolism in H. axyridis larvae. The results showed that injection of dsGLUT1 and dsGLUT4 significantly affected the expression of TPS, TRE2, TRE2-like and other genes, and it was found that the contents of glycogen, glucose, trehalose and other carbohydrates decreased after interfering with the expression of GLUT1 and GLUT4 in larvae. It indicates that GLUT1 and GLUT4 play a certain regulatory role in the reproduction of H. axyridis larvae. The results of the study will help provide theoretical support for further research on the relationship between key gene expression and energy metabolism of H. axyridis larvae in the future and provide technical guidance and reference for rational use of H. axyridis in biological control.

Key words: Harmonia axyridis, artificial feed, glucose, glucose transporter, RNA interference, energy metabolism

CLC Number:

S476.2

GE Xinzhu, LI Yan, GU Shan, WANG Shasha, CHAO Lei, CAI Wenjie, LI Hangyu, FANG Yinjie, WANG Shigui, TANG Bin. Effects of GLUT Gene Expression on Energy Metabolism in Harmonia axyridis[J]. Chinese Journal of Biological Control, 2023, 39(1): 98-110.

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