Isolation and Identification of Burkholderia cenocepacia YM12 and Its Control Effect on Cucumber Soft Rot Disease

doi:10.16409/j.cnki.2095-039x.2024.02.059

Abstract

Abstract: Soft rot of cucumber is a bacterial disease caused by Pectobacterium brasiliense. The disease is serious and difficult to control. In order to screen biocontrol microorganisms with high antibacterial effect, a strain YM12 with significant inhibitory effect on P. brasiliense was isolated from cucumber rhizosphere soil. Through morphological observation, physiological and biochemical determination and multi-gene phylogenetic tree analysis, the strain was identified as Burkholderia cenocepacia. Analysis of antibiotic synthesis-related genes showed that strain YM12 might also produce Pyrrolnitrin and Pyhluteorin. Analysis of biocontrol traits characteristics confirmed that YM12 had the ability to produce phosphatase protease and pyoverdine. The strain had antagonistic effects on 5 pathogenic bacteria and 3 pathogenic fungi by antimicrobial spectrum analysis. Pot experiment showed that strain YM12 had obvious control effect on cucumber soft rot, with the efficacy of 62.84%.In summary, strain YM12 is the first reported B. cenocepacia with biocontrol effect on bacterial soft rot, which has high biocontrol research value and development and application potential.

Key words: Burkholderia cenocepacia, cucumber bacterial soft rot, antibacterial activity, biological control

CLC Number:

S476

WEI Xinchen, WANG Qiao, SHI Bin, XIE Xuewen, SHI Yanxia, CHAI Ali, LI Baoju, LI Lei. Isolation and Identification of Burkholderia cenocepacia YM12 and Its Control Effect on Cucumber Soft Rot Disease[J]. Chinese Journal of Biological Control, 2024, 40(5): 1088-1098.

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