Identification and Biocontrol Effect of Bacillus amyloliquefaciens YW-2-6 against Alternaria alternata

doi:10.16409/j.cnki.2095-039x.2022.08.005

Abstract

Abstract: To obtain effective biocontrol resources against Alternaria alternata, the antagonistic strains against A. alternata were isolated and screened from tobacco rhizosphere soil by methods of dilution separation and plate confrontation.Morphological observation, physiological and biochemical characteristics and 16S rDNA sequence analysis were performed to identify the antagonistic strains, and their growth-promoting effect on tobacco plants was also determined. In addition, the IAA production ability of the strains was verified, and the effect of the fermentation broth of biocontrol bacteria on tobacco brown spot was evaluated in the field. Consequently, a total of five antagonistic bacteria against tobacco brown spot were screened from the 65 isolated strains. Strain YW-2-6 had the best antagonistic effect against tobacco brown spot, with the inhibition rate of 71.32 %. Furthermore, strain YW-2-6 was preliminarily identified as Bacillus amyloliquefaciens. It showed obvious antagonistic effects against Fusarium graminearum, Phytophthora capsica and other pathogens. The control efficacy of YW-2-6 fermentation broth on tobacco brown spot was 56.93 %, and the strain YW-2-6 had prominent growth-promoting effect on tobacco plants. Therefore, strain YW-2-6 showed good biocontrol potential and development prospect.

Key words: tobacco brown spot, biological control, antagonistic effect, Bacillus amyloliquefaciens

CLC Number:

S476

XIAO Zhipeng, LI Lingling, MU Tingting, SHAN Xuehua, LU Feng, TANG Qianjun, LIU Tianbo. Identification and Biocontrol Effect of Bacillus amyloliquefaciens YW-2-6 against Alternaria alternata[J]. Chinese Journal of Biological Control, 2022, 38(6): 1598-1607.

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