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Chinese Journal of Biological Control ›› 2023, Vol. 39 ›› Issue (1): 212-220.DOI: 10.16409/j.cnki.2095-039x.2023.02.008

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Screening and Identification of Bacillus safensis ZG6 and Its Biocontrol Potential against Gray Blight on Camellia sinensis

LIU Jiaojiao, YAN Zhewei, ZHANG Yiju, FU Min, ZHANG Lixin   

  1. Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes/College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
  • Received:2022-07-22 Online:2023-02-08 Published:2023-02-21

Abstract: Tea gray blight is a severe disease that occurred in tea plantings in China, causing significant losses to tea yield and quality. In order to obtain effective biocontrol strains against tea gray blight, an endophytic bacterium ZG6 with strong antagonistic activity against the pathogen Pseudopestalotiopsis sp. DSP-6 was isolated and screened from tea roots in Hefei. The strain ZG6 inhibited mycelial growth by dual culture assay, with an inhibition rate of 65.38%. Based on colony morphology, physiological and biochemical characteristics, partial nucleotide sequences of 16S rRNA and gyrB gene, strain ZG6 was identified as Bacillus safensis. The strain ZG6 had a broad-spectrum inhibition ability against Sclerotinia sclerotiorum, Bipolaris maydis, and Cytospora mandshurica, with inhibition rates of 78.82%, 64.71% and 61.82%, respectively. The enzymatic test revealed that strain ZG6 produced cellulase and protease in the metabolic process. The fermentation broth, fermentation filtrate and suspension of strain ZG6 showed control efficacy over 69.9% against gray blight on the leaf of the tea detached branch. In summary, B. safensis ZG6 could be a potential biocontrol agent for the management of tea gray blight.

Key words: tea plant, endophytic bacteria, gray blight, biological control

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