Isolation,Identification and Control Effect of Biocontrol Bacteria against Ginseng Black Spot Disease

doi:10.16409/j.cnki.2095-039x.2022.02.033

Abstract

Abstract: An antagonistic bacterial strain YJ 3-7 was screened from the rhizosphere soil of ginseng by plate confrontation method using Alternaria panax as indicators.Combined with cell morphology,16S rDNA and gyrA gene sequence alignment analysis,the strain YJ 3-7 was identified as Bacillus velezensis.Fermentation broth,bacterial suspension and fermentation liquid without bacteria of strain YJ 3-7 all had inhibitory effects on the growth of A.panax,and the inhibition rates were 98.76%,98.27% and 70.62%,respectively.In vitro leaf experiment and pot experiment showed that YJ 3-7 fermentation broth could control ginseng black spot disease,with the control efficacies of 63.24% and 67.92%,respectively.The pot control effect of YJ 3-7 fermentation broth was slightly lower than that of carbendazim (72.81%).In addition,strain YJ 3-7 could effectively inhibit the growth of other 8 plant pathogens.The above results show that B.velezensis YJ 3-7 has broad-spectrum antagonistic effect,and obvious control effect on ginseng black spot disease,which laid a foundation for the development of bio-control agents against ginseng black spot disease.

Key words: ginseng black spot, Bacillus velezensis, biological control

CLC Number:

ZHANG Ning, ZHANG Jingjing, LI Yashu, LI Ranqi, HOU Wei, QU Zhengyi, LI Yali, ZHENG Peihe. Isolation,Identification and Control Effect of Biocontrol Bacteria against Ginseng Black Spot Disease[J]. Chinese Journal of Biological Control, 2022, 38(5): 1308-1315.

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