Screening, Identification and Biocontrol Effect of Antagonistic Strain against Fusarium oxysporum f. sp. cucumerinum

doi:10.16409/j.cnki.2095-039x.2023.02.006

Abstract

Abstract: In order to obtain biocontrol strains with high antagonistic activity against Fusarium oxysporum f. sp. cucumerinum (FOC), antagonistic bacterial strains against FOC were isolated and screened from healthy cucumber plants rhizosphere soils by soil dilution plating and plate confrontation growth methods. The strain with the strongest antagonistic ability was identified based on morphological characteristics, physiological and biochemical indexes, whole cell fatty acids in combination with 16S rRNA and gyrB gene sequence homology analysis. Meanwhile, the inhibitory effects of antagonistic strain cell-free fermentation filtrate on mycelial growth and spore germination of FOC were determined, and the control effects of fermentation liquid on cucumber Fusarium wilt in greenhouse potted and field experiments were further evaluated. The results showed that 23 strains of bacteria with inhibitory activity against FOC were primarily screened out, and one strain FJ17-4 which exhibited the strongest inhibition capability was identified as Bacillus velezensis. FJ17-4 could seriously disrupt FOC mycelium growth and caused abnormal phenomena such as mycelial deformity, distortion, swelling, shrinkage and curling. The inhibition rates of 10% cell-free fermentation filtrate of FJ17-4 on mycelial growth and spore germination were 70.96% and 85.40%, respectively. In addition, the control effects of 50 fold fermentation liquid, living bacterial cell suspension and sterile filtrate FJ17-4 of strain on cucumber Fusarium wilt were 70.20%, 58.87% and 47.80% in potted experiment, and 69.53%, 58.46% and 36.12% in field trials, respectively. In summary, FJ17-4 could effectively inhibit FOC and exhibited significantly biological control effect on cucumber Fusarium wilt, and had broad application prospects.

Key words: cucumber Fusarium wilt, Fusarium oxysporum f. sp. cucumerinum, biocontrol, Bacillus velezensis

CLC Number:

S476

LAN Chengzhong, GAN Lin, DAI Yuli, LIU Xiaofei, YANG Xiujuan, JIANG Junxi. Screening, Identification and Biocontrol Effect of Antagonistic Strain against Fusarium oxysporum f. sp. cucumerinum[J]. Chinese Journal of Biological Control, 2023, 39(1): 184-193.

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