Research of Inhibitory Effect of Total Flavones from Solidago canadensis (Linnaeus) on Growth of Fusarium oxysporum

doi:10.16409/j.cnki.2095-039x.2023.02.023

Abstract

Abstract: To investigate the inhibitory effect of total flavonoids from Solidago canadensis (Linnaeus) on the growth of Fusarium oxysporum, seven treatments of total flavonoids (0—18 mg/mL) extracted from inflorescence of S. canadensis by ultrasonic method were set, and the growth, physiological indexes of F. oxysporum f. sp. Melonis FOM as well as field control effect were determined. The results showed that the inhibition rate of spore germination of FOM reached 100.00%, and hypha broken with distortion, the value of OD₆₅₀ (0.18) and pH (3.5) were the smallest under the treat of 18 mg/mL total flavonoids. However, its electrical conductivity was up to 1.3 times of the control. Moreover, the activities of superoxide dismutase (SOD) and catalase (CAT) in FOM increased first and then decreased after 24 h under gradient concentration of total flavonoids of S. canadensis, and the activities of both enzymes were reduced to 4.69 and 19.00 U/mL at 48 h in 18 mg/mL treatment, respectively. The results of the field experiment showed that the number of dead plants of the test Sugua Cucumis melo had a negative effect on the treatment concentration of total flavonoids of S. canadensis, indicating that the higher concentration of total flavones from S. canadensis irrigated the soil repeated planting could effectively inhibit the growth of FOM and reduce its pathogenicity. Therefore, this study can provide theoretical reference for the rational development of S. canadensis resources and the expansion of new green plant-derived control agents to FOM.

Key words: Solidago canadensis, total flavonoids, Fusarium oxysporum, growth and physiology

CLC Number:

S476

LIU Ling, LIU Haitao, YANG Zongsai, ZHANG Chaoyang, ZHENG Binqing, LIN Beijia, LIANG Jingjing, WANG Zhenglong. Research of Inhibitory Effect of Total Flavones from Solidago canadensis (Linnaeus) on Growth of Fusarium oxysporum[J]. Chinese Journal of Biological Control, 2023, 39(3): 607-614.

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