Effects of Temperature on Growth and Pathogenicity of Two Strains of Xenorhabdus bovenii

doi:10.16409/j.cnki.2095-039x.2023.02.058

Abstract

Abstract: To determine the influence of temperature on the growth and virulence of the symbiotic bacteria, as well as the optimal culture conditions, the experiment was conducted by plate and fermentation broth culture method under different temperature treatment conditions. Two strains of X. bovenii 0641TY and 0657L were cultured to study their growth rate and their hematotoxicity and gastrotoxicity to G. mellonella. The results showed that the average colony diameters of the X. bovenii 0641TY and 0657L with plate culture were 1653.34 and 834.84 μm respectively at 30 ℃. The two strains reached logarithmic growth stage at 6 and 12 h respectively at 30 ℃, and the growth rate was significantly higher than that at other temperatures.. The two strains cultured at 30 ℃ showed high gastrotoxicity to the larvae, and the corrected mortality rate reached 100% after 72 h of infection, while the hematotoxicity was weak, the mortality rate was 50% and 46.6% respectively after 96 h of infection.Therefore, 30 ℃ was beneficial to the growth and virulence of the two strains of X. boveni.

Key words: Xenorhabdus bovenii, temperature, biological characteristics, virulence, Galleria mellonella

CLC Number:

S476

ZHANG Wende, LI Xitong, QIAN Xiuijuan. Effects of Temperature on Growth and Pathogenicity of Two Strains of Xenorhabdus bovenii[J]. Chinese Journal of Biological Control, 2023, 39(6): 1455-1462.

References

[1] Toepfer S, Kurtz B, Kuhlmann U. Influence of soil on the efficacy of entomopathogenic nematodes in reducing Diabrotica virgifera in maize[J]. Journal of Pest Science, 2010, 83(3): 257-264.
[2] Bal H K, Grewal P S. Lateral dispersal and foraging behavior of entomopathogenic nematodes in the absence and presence of mobile and non-mobile hosts[J]. Public Library of Science, 2015, 10(6): e0129887.
[3] 杨秀芬, 杨怀文. 昆虫病原线虫的致病机理[J]. 中国生物防治, 1998, 14(4): 38-42.
[4] 王立婷, 丘雪红, 吴春艳, 等. 昆虫病原线虫和共生细菌定殖关系的研究进展[J]. 环境昆虫学报, 2013, 35(2): 232-241.
[5] 丘雪红, 曹莉, 韩日畴. 昆虫病原线虫共生细菌共生性的分子生物学研究进展[J]. 昆虫知识, 2010, 47(5):824-833.
[6] 游娟, 梁世中, 韩日畴. 昆虫病原线虫共生细菌共生性与病原性的研究进展[J]. 中国生物防治, 2006，22(3): 168-173.
[7] 李立涛. 嗜线虫致病杆菌的杀虫机理及其水悬浮剂的研究[D]. 保定: 河北农业大学, 2011.
[8] 刘奇志, 张丽娟, 王玉柱, 等. 昆虫病原线虫(Steinernema longicaudum BPS品系)生物学测定[J]. 中国农学通报, 2009, 25(2): 236-239.
[9] 张彩铃, 王志敏, 王思雨, 等.昆虫病原线虫对暗黑鳃金龟的致病力[J/OL].中国生物防治学报:1-10, 2023, doi.org/10.16409/j.cnki.2095-039x. 2022.03.025.
[10] 段秀峰, 林茂松, 李红梅, 等. 伯氏致病杆菌(Xenorhabdus bovienii BE)杀虫活性物质的分离和生物测定[J].江苏农业学报, 2009, 25(1):59-67.
[11] 孟亚莉, 丛斌, 王欢, 等. 昆虫病原线虫共生菌对玉米螟幼虫的杀虫活性[J]. 沈阳农业大学学报, 2004(2): 97-100.
[12] Tobias N J, Wolff H, Djahanschiri B, et al. Natural product diversity associated with the nematode symbionts Photorhabdus and Xenorhabdus[J]. Nature Microbiology, 2017, 2(12): 1676-1685
[13] Muangpat P, Suwannaroj M, Yimthin T, et al. Antibacterial activity of Xenorhabdus and Photorhabdus isolated from entomopathogenic nematodes against antibiotic-resistant bacteria[J]. Public Library of Science, 2020, 15(6): e0234129.
[14] 肖尧, 杨秀芬, 杨怀文.致病杆菌和发光杆菌抗菌代谢产物研究进展[J]. 中国生物防治学报, 2011, 27(4): 553-558.
[15] Bertoloni M S, Bashey F. Trade-off between reproductive and anticompetitor abilities in an insect-parasitic nematode-bacteria symbiosis[J]. Ecology and Evolution, 2018, 8(22): 10847-10856.
[16] 方香玲, 张武岗, 易晓华, 等. 三株昆虫病原线虫共生菌胞外产物的抑菌活性[J]. 中国生物防治, 2008(4): 354-358.
[17] Guo W X, Yan X, Han R C, et al. Increased efficacy of entomopathogenic nematode-insecticide combinations against Holotrichia oblita (Coleoptera: Scarabaeidae)[J]. Journal of Economic Entomology, 2017, 110(1): 41-51.
[18] Sarciaux M, Pantel L, Midrier C, et al. Total synthesis and structureactivity relationships study of odilorhabdins，a new class of peptides showing potent antibacterial activity[J]. Journal of Medicinal Chemistry, 2018, 61(17): 7814-7826.
[19] Liu J, Nan G Z, Zhang J, et al. Expression and characterization of two chitinases with synergistic effect and antifungal activity from Xenorhabdus nematophila[J]. World Journal of Microbiology and Biotechnology, 2019, 35(7): 106.
[20] Xi X D, Lu X, Zhang X, et al. Two novel cyclic depsipeptides Xenematides F and G from the entomopathogenic bacterium Xenorhabdus budapestensis[J]. The Journal of Antibiotics, 2019, 72(10): 736-743.
[21] Dreyer J, Rautenbach M, Booysen E, et al. Xenorhabdus khoisanae SB10 produces Lys-rich PAX lipopeptides and a xenocoumacin in its antimicrobial complex[J]. BMC Microbiology, 2019, 19(1): 132.
[22] Zhang X, Machado R A, Doan C V, et al. Entomopathogenic nematodes increase predation success by inducing cadaver volatiles that attract healthy herbivores[J]. Elife, 2019, 8: e46668.
[23] Zhao L, Awori R M, Kaiser M, et al. Structure, biosynthesis, and bioactivity of photoditritide from Photorhabdus temperate Megl[J]. Journal of Natural Products, 2019, 82(12): 3499-3503.
[24] Imai Y, Meyer K J, Iinishi A, et al. A new antibiotic selectively kills gram-negative pathogens[J]. Nature, 2019, 576(7787): 459-464.
[25] Booysen E, Rautenbach M, Stander M A, et al. Profiling the production of antimicrobial secondary metabolites by Xenorhabdus khoisanae J194 under different culturing conditions[J]. Frontiers in Chemistry, 2021, 9: 626653.
[26] 张奎花, 钱秀娟, 刘长仲. 影响昆虫病原线虫共生菌发酵液杀虫活性相关因子研究[J]. 植物保护, 2013, 39(5):172-176.
[27] Shokoofeh K, Javad K, Albrecht M K. New insight into the management of the tomato leaf miner, Tuta absoluta (Lepidoptera: Gelechiidae) with entomopathogenic nematodes[J]. Journal of Economic Entomology, 2018, 111(1): 112-119.
[28] Yan X, Zhao G Y, Han R C. Integrated management of chive gnats (Bradysia odoriphaga Yang ＆ Zhang) in chives using entomopathogenic nematodes and low-toxicity insecticides[J]. Insects, 2019, 10(6): 161.
[29] Ali F, Wharton D A. Cold tolerance abilities of two entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis bacteriophora[J]. Cryobiology, 2013, 66(1): 24-29.
[30] Ffrench-Constant R H, Bowen D J. Novel insecticidal toxins from nematode- symbiotic bacteria[J]. Cellular and Molecular Life Science, 2001, 57(5): 828-833.
[31] 付俊瑞, 刘奇志, 李星月. 昆虫病原线虫异小杆属新种Heterorhabditis beicherriana共生菌的分离及致病性[J]. 中国生物防治学报, 2018, 34(1): 133-140.
[32] 孟亚莉. 昆虫病原线虫共生细菌高毒力菌株的筛选及杀虫活性物质的研究[D]. 沈阳: 沈阳农业大学, 2004.
[33] Ensign J C, Bowen D J, Bin trim S. Crystalline inclusion proteins and an insecticidal toxin of Xenorhabuds luminescens strain NC-19[A]//Proceedings and Abstracts of 5th International Colloquium on Invertebrate Pathology and Society for Inverteb rate Pathology and Microbial Control, Adel aide, Australia, 1990: 218.
[34] 王勤英, 南宫自艳, 陆秀君, 等. 嗜线虫致病杆菌HB310菌株杀虫蛋白的纯化及活性鉴定[J]. 昆虫学报, 2005(3): 353-358.
[35] Bowen D, Rocheleau T A, Blackburn M, et al. Novel insecticidal toxins from the bacterium Photorhabdus luminescens[J]. Science, 1998, 280(26): 2129-2132.
[36] 王立霞, 潘映红, 杨怀文, 等. 高毒力昆虫病原线虫共生细菌菌株的筛选及其杀虫活性的检测[J]. 昆虫学报, 2000(S1): 98-103.
[37] 张文波. 昆虫病原线虫共生菌SN52菌株的次生代谢产物和生物活性研究[D]. 沈阳: 沈阳农业大学, 2017.