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Chinese Journal of Biological Control ›› 2023, Vol. 39 ›› Issue (5): 1194-1203.DOI: 10.16409/j.cnki.2095-039x.2023.02.044

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Research of Biocontrol Actinomycete ZZ-9 Granules and Its effect on cucumber Rhizoctonia Rot

ZHANG Yang, WEI Lanjing, CHEN Junhong, HEI Yaya, XUE Yingyu   

  1. College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China
  • Received:2022-11-11 Online:2023-10-08 Published:2023-10-25

Abstract: Streptomyces rochei ZZ-9 is a biocontrol strain that with broad-spectrum antifungal activity. The mycelium growth rate method was utilized in this work to screen carriers, stabilizers, disintegrants, adhesives, and wetting agents. The optimal formula of Streptomyces rochei ZZ-9 granules were: the ratio of bacterial solution and attapulgite was 2:5, calcium carbonate 1%, magnesium chloride 2%, polyvinyl alcohol 1%, sodium dodecylbenzene sulfonate 0.5%. The effective viable number of ZZ-9, particle size, moisture content, disintegration time in water, pH value and hardness were 1.19×108cfu/g, 93.5%, 2.5%, 96 s, 6.35 and 10%. The results of pot experiment showed that the optimal controlling effect of cucumber Rhizoctonia rot was 76.92% after application of ZZ-9 granule with 3 g/pot. In addition,the Streptomyces rochei ZZ-9 granules presented significant promoting effects in pot experiment. Compared with the control, the germination rate, plant height, root length and fresh weight of cucumber seedlings were increased by 9.34%, 29.52%, 27.54% and 22.96% after application of Streptomyces rochei ZZ-9 granules, respectively.

Key words: Streptomyces rochei, cucumber, Rhizoctonia solani, granule, controlling effect

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