Antifungal Activity of Volatile Organic Compounds from Bacillus amyloliquefaciens XJ5 and Control Effect against Monilinia fructigena

doi:10.16409/j.cnki.2095-039x.2020.04.018

Abstract

Abstract: Volatile organic compounds (VOCs) of Bacillus amyloliquefaciens XJ5 have good antifungal activity against various pathogenic fungi such as Monilinia fructigena. The VOCs produced by strain XJ5 were detected by SPME-GC-MS, their antifungal activities were determined by two-sealed-base-plates method, and the control effects against M. fructigena were evaluated in vitro. The results indicated that inhibition rate of XJ5 VOCs against nine pathogenic fungi were ranged between 47.9% and 84.8%, and the highest inhibition rate against M. fructigena was 84.8%. SPME-GC-MS analysis and antifungal activity determination showed that the major VOCs produced by strain XJ5 were Dodecanal, and the major VOCs with antifungal activity were 2-Nonanol, 2-Ethyl-1-hexanol and 2-Undecanol. The EC₅₀ value of 2-Nonanol inhibiting M. fructigena was 3.43 μg/mL. In vitro fumigation with XJ5 bacteria suspension and 2-Nonanol can effectively inhibited the mycelial growth of M. fructigena and the lesion development. The fumigation control efficacies of XJ5 bacteria suspension and 2-Nonanol against M. fructigena were 14.4%~71.2% and 66.4%~97.6%, respectively, significantly different from those of the control.

Key words: Bacillus amyloliquefaciens, volatile organic compounds, SPME-GC-MS, antifungal activity, control effectin in vitro

CLC Number:

S476

GUO Jiexin, ZHANG Yuming, ZHU Honglei, REN Lu, ZHANG Baojun. Antifungal Activity of Volatile Organic Compounds from Bacillus amyloliquefaciens XJ5 and Control Effect against Monilinia fructigena[J]. Chinese Journal Of Biological Control, 2020, 36(4): 575-580.

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