Screening of Biocontrol Bacillus spp. and Its Suppression Efficacy on Grape Gray Mold

doi:10.16409/j.cnki.2095-039x.2022.02.016

Abstract

Abstract: Biocontrol strains were isolated from healthy grape rhizosphere soil collected from Xinjiang, Shandong, Hebei provinces. Four antagonistic strains showed good biocontrol activities against Botrytis cinerea on grape leaves and fruit by the plate test. Based on morphology and phylogenetic tree analysis, the strains（BF1-1、BF2-1、BF3-1 and BF4-1）were identified as Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus cereus, respectively. The control efficacies of fertilizer made from the four strains were between 66.50%～85.68% in the field trial.

Key words: grape gray mold, biological control, Bacillus spp., control effect

CLC Number:

S476

FU Liyuan, CAI Ruijie, FENG Zhimin, SONG Xinna, ZHU Yingbo, SHI Fengyu, LIU Jianbin. Screening of Biocontrol Bacillus spp. and Its Suppression Efficacy on Grape Gray Mold[J]. Chinese Journal of Biological Control, 2022, 38(2): 440-446.

References

[1] Zhang H Y, Godana E A, Yuan S, et al. Biological control as an alternative to synthetic fungicides for the management of grey and blue mould diseases of table grapes: a review[J]. Critical Reviews in Microbiology, 2020, 46: 4, 450-462.
[2] 王忠跃. 葡萄健康栽培与病虫害防控[J]. 果农之友, 2020(8): 51.
[3] 邓庭和. 新疆塔额盆地设施葡萄灰霉病发生规律及防控技术的研究[D]. 石河子: 石河子大学, 2015.
[4] 沈建生, 滕元文, 王华新, 等. 施佳乐、扑海因、速克灵单用及混用防治葡萄灰霉病田间药效试验[J].中国南方果树, 2005, 34(6): 66-67.
[5] 韦发才. 防治葡萄灰霉病药剂筛选试验[J]. 中国南方果树, 2005(6): 77-78.
[6] Zhang J, Wang Y, Du Z L, et al. Screening, identification and antagonistic effect of antagonistic bacteria JTFM1001 against aflatoxin contamination in corn[J]. Oil Crop Science, 2021, 6(1): 1-7.
[7] Elad Y. Trichoderma harzianum T39 Preparation for Biocontrol of Plant Diseases-Control of Botrytis cinerea, Sclerotinia sclerotiorum and Cladosporium fulvum[J]. Biocontrol Science and Technology, 2010, 10: 4, 499-507.
[8] 童蕴慧, 郭桂萍, 徐敬友, 等. 拮抗细菌对番茄植株抗灰霉病的诱导[J]. 中国生物防治, 2004, 20(3): 187-189.
[9] 杨春林, 席亚东, 谢华蓉, 等. 哈茨木霉Th-30几丁质酶的生产条件及对灰霉病菌的拮抗作用[J]. 植物保护学报, 2009, 36(4): 295-300.
[10] 荆卓琼, 郭致杰, 徐生军, 等. 解淀粉芽胞杆菌HZ-6-3的筛选鉴定及其防治番茄灰霉病效果的评价[J]. 草业学报, 2020, 29(2): 31-41.
[11] 叶涛, 刘敏, 任哲昕, 等. 番茄灰霉菌拮抗细菌的筛选鉴定及评价[J]. 浙江师范大学学报(自然科学版), 2020, 43(1): 60-65.
[12] 张飞官, 高雅慧, 任慧爽, 等. 桑疫病病原拮抗菌的分离、鉴定及发酵条件优化[J]. 微生物学报, 2013, 53(12): 1285-1294.
[13] 乔淼, 陆桥君. 一起生活饮用水污染蜡样芽胞杆菌的检测与分析[J]. 江苏预防医学, 2019, 30(6): 679-680.
[14] Oda M, Yokotani A, Hayashi N, et al. Role of sphingomyelinase in the pathogenesis of Bacillus cereus infection[J]. Biological and Pharmaceutical Bulletin, 2020, 43(2): 250-253.
[15] 梁斌, 何月悦. 一起蜡样芽胞杆菌引起食物中毒事件调查分析[J]. 社区医学杂志, 2020, 18(16): 1122-1124.
[16] 陈哲, 黄静, 赵佳, 等. 番茄灰霉病病原菌分离鉴定及拮抗菌筛选[J]. 生物技术通报, 2017, 33(8): 81-87.
[17] 柴庆凯, 张斌, 常若葵, 等. 解淀粉芽胞杆菌LJ02对黄瓜抗灰霉病菌的生防效果及其诱导抗性机理的初步研究[J]. 植物病理学报, 2019, 49(6): 828-835.
[18] 黄静, 赵佳, 陈哲, 等. 抗草莓灰霉病的芽胞杆菌CM3的分离与筛选[J]. 北方园艺, 2016(11): 113-116.
[19] 罗琳, 王其慧, 赵海霞, 等. 葡萄灰霉病生防菌株的筛选及其拮抗机理初探[J]. 中国酿造, 2017, 36(4): 93-98.
[20] 王良. 地衣芽胞杆菌的应用现状及展望[J]. 生物技术世界, 2013, 10(4): 172.
[21] 朱薇. 桃褐腐病菌的生物学和地衣芽胞杆菌W10的防病机理研究[D]. 扬州: 扬州大学, 2019.
[22] 陈刘军, 俞仪阳, 王超, 等. 蜡质芽胞杆菌AR156防治水稻纹枯病机理初探[J]. 中国生物防治学报, 2014, 30(1): 107-112.
[23] 王红丽, 善文辉, 胡海瑶, 等. 生防菌混合接种对葡萄灰霉病菌的防治效果[J]. 中国生物防治学报, 2020, 36(2): 265-271.
[24] 贺惠文, 纪兆林, 徐敬友, 等. 地衣芽胞杆菌W10与化学药剂复配对桃褐腐病菌抑制活性的增效作用[J]. 亚热带植物科学, 2017, 46(4): 391-393.