Development of Powder for Dry Seed Treatment based on Bacillus amyloliquefaciens PHODG36 and Its Control Efficacy on Cotton Verticillium Wilt

doi:10.16409/j.cnki.2095-039x.2024.02.054

Abstract

Abstract: The combination application of microbial agents and plant residues could effectively reduce the incidence of plant diseases. In order to further develop the application potential of broccoli residues in the control of soil-borne diseases, a powder for dry seed treatment formulation (DS) for cotton Verticillium wilt was developed based on Bacillus amyloliquefaciens PHODG36 as the active ingredient, and dry broccoli residues powder as the carrier in the study. By optimizing the ratio of carrier, additives, and technical concentrate (TK), the optimal formula of 10 billion CFU/g B. amyloliquefaciens DS was established as follows: 20% strain PHODG36 TK, 77% broccoli residues dry powder, 1% dispersant sodium salt of polynaphthalene sulphonic acid (NNO), and 2% wetting agent sodium dodecyl benzene sulfonate (SDBS). The results showed that PHODG36 DS had a light yellow appearance and no agglomeration, with an average particle size of 37.65 μm, and there was no caking and sticking after 14 days of storage at (54±2) ℃. The results showed that 10 billion CFU/g PHODG36 DS had a promoting effect on cotton plant height and biomass, and the control efficacy on CVW reached 73.03% in greenhouse experiments, 61.60% in field plot experiments. Field trial demonstrations in different 7 regions (in Hebei and Shandong provinces) showed that the control efficacy on CVW ranged from 61.08% to 74.8%，and the number of bolls per plant increased from 3.38% to 27.42%. Comprehensive analysis showed that 10 billion CFU/g PHODG36 DS can effectively reduce the incidence of CVW and increase the number of bolls per plant. Therefore, it had broad application prospects for cotton production in the Yellow River Basin cotton region of China.

Key words: broccoli residue dry powder, Bacillus amyloliquefaciens, seed treatment, cotton Verticillium wilt, microbial agents

CLC Number:

S476

ZHAO Weisong, GUO Qinggang, LU Xiuyun, GOU Jianjun, ZHAO Ming, LI Shezeng, MA Ping. Development of Powder for Dry Seed Treatment based on Bacillus amyloliquefaciens PHODG36 and Its Control Efficacy on Cotton Verticillium Wilt[J]. Chinese Journal of Biological Control, 2024, 40(6): 1310-1318.

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