Synergistic Effect of Zhongshengmycin and Natamycin on Tomato Gray Mold

doi:10.16409/j.cnki.2095-039x.2022.04.005

Abstract

Abstract: In order to improve the control effect of existing bio-pesticides on tomato gray mold,the inhibitory activities of mixtures containing Zhongshengmycin and Natamycin on mycelial growth and conidial germination of Botrytis cinerea were investigated,and the synergistic effect and appropriate ratio of the two pesticides were determined.The mixtures of Zhongshengmycin and Natamycin showed synergistic effect on inhibition of hyphae growth when their weight ratios were at 9:1 and 1:1,with the synergistic coefficient of 3.6348 and 1.7145,respectively.Synergistic effect was also expressed in inhibition of the conidial germination when their weight ratios were at 9:1,7:3 and 1:1,with the synergistic coefficient of 1.7268,1.6335 and 1.5336,respectively.Field experiments showed that the 500 x dilution of 3% wettable compound powder of Zhongshengmycin and Natamycin (9:1) performed good effects on tomato gray mold on leaves and fruits,with the control efficacies of 81.04% and 78.48%,respectively,significantly higher than that of individual ingredient.The above results showed that the mixture of Zhongshengmycin and Natamycin in proper proportion could effectively improve the control effect on tomato gray mold.

Key words: Botrytis cinerea, Zhongshengmycin, Natamycin, synergistic effect

CLC Number:

S476

JI Lindong, MA Chao, XU Jun, ZHAO Yijun, ZHANG Yanhui, DUAN Xiaoli, YANGYang, CHEN Chang. Synergistic Effect of Zhongshengmycin and Natamycin on Tomato Gray Mold[J]. Chinese Journal of Biological Control, 2022, 38(4): 874-879.

References

[1] 董金皋.农业植物病理学2版[M].北京:中国农业出版社, 2007, 391-393.
[2] 吴心勤,赵康涛,陈明中.某中生菌素的急性毒性评价[J].海峡预防医学杂志, 2009, 15(5):48-49.
[3] Yafeng R, Dongxue L, Shilong J, et al. Integration of transcriptomic and proteomic data reveals the possible action mechanism of the antimicrobial zhongshengmycin against didymella segeticola, the causal agent of tea leaf spot[J]. Phytopathology, 2021, 111(12):2238-2249.
[4] 陈方圆,戴久竣,徐家延,等.纳他霉素抑菌机制及在食品保鲜中的应用研究进展[J].食品科技, 2021, 46(9):47-51.
[5] 张鹏.纳他霉素对灰葡萄孢的活性及作用机理研究[D].泰安:山东农业大学, 2008.
[6] 姚亚丽,潘忠成,郑鹏飞,等.中生菌素在植物病害防治上的应用研究进展[J].衡阳师范学院学报, 2019, 40(6):93-98.
[7] 凌金锋,周娟,彭埃天,等.吡唑醚菌酯与代森锰锌对两种果树病原菌的联合毒力[J].植物保护, 2016, 42(5):246-250.
[8] 农业部农药检定所. NY/T 1156.6-2006农药室内生物测定试验准则杀菌剂第6部分[M]//混配的联合作用测定.北京:中国农业出版社, 2006, 1-3.
[9] NY/T 1156.1-2006,农药室内生物测定试验准则杀菌剂第1部分:抑制病原真菌孢子萌发试验凹玻片法[S].
[10] 罗大成. 17%吡唑醚菌酯·氟苯醚酰胺悬浮剂研制及对番茄灰霉病田间防治效果[D].杨凌:西北农林科技大学, 2021.
[11] 陈凤平,韩平,张真真,等.啶菌噁唑对番茄灰霉病菌的抑菌作用研究[J].农药学学报, 2010, 12(1):42-48.
[12] 周明国,叶钟音,刘经芬.南京市郊灰霉菌对苯并咪唑类杀菌剂田间抗性的检测[J].南京农业大学学报, 1987(2):53-58.
[13] Fatima A, Najwa B, Mohamed A, et al. In field control of botrytis cinerea by synergistic action of a fungicide and organic sanitizer[J]. Journal of Integrative Agriculture, 2018, 17(6):1401-1408.
[14] 张亚,刘青,刘双清,等.啶酰菌胺和咯菌腈复配对草莓灰霉病菌的室内毒力测定及田间防效[J].中国蔬菜, 2018(2):53-57.
[15] 黄海,张鑫,邹杭,等.番茄灰霉病菌室内毒力测定及药效试验[J].西北农林科技大学学报(自然科学版), 2015, 43(2):184-190.