Screening of Entomopathogenic Nematodes with High Virulence to Loxostege sticticalis

doi:10.16409/j.cnki.2095-039x.2026.05.016

Abstract

Abstract: To identify entomopathogenic nematode(EPN) strains with potential biological control of Loxostege sticticalis larvae, the virulence of six nematode strains to third-instar larvae was evaluated under laboratory conditions. In addition, the susceptibility of larvae at different instars, as well as the effects of application dose and environmental temperature on nematode pathogenicity, was investigated. The results showed that the Steinernema feltiae SFSN and S. carpocapsae ScALL strains exhibited the highest virulence, causing larval mortality exceeding 90%. Subsequently, analysis of sensitivity of L. sticticalis larvae to the two nematode strains at different developmental stages showed that the third-instar larvae were more susceptible to infection than the fourth-and fifth-instar larvae. Single-factor experiments further indicated that the optimal application doses were 60 IJs per larva for S. feltiae SFSN and 80 IJs per larva for S. carpocapsae ScALL. The optimal temperature range for infection was 21—25 ℃. These results suggest that S. feltiae SFSN and S. carpocapsae ScALL have considerable potential for the biological control of L. sticticalis.

Key words: Loxostege sticticalis, entomopathogenic nematode, virulence

CLC Number:

S476

LUO Jing, LI Shuohan, WANG Zhimin, YIN Jiao, CAO Yazhong, FAN Dong, LI Kebin. Screening of Entomopathogenic Nematodes with High Virulence to Loxostege sticticalis[J]. Chinese Journal of Biological Control, 2026, 42(3): 759-765.

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