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Analysis of Inhibitiory Active Substances of Penicillium bilaiae Strain 47M-1 Based on Non-Targeted Metabolomics ZHAO Xinbei, NI Yunxia, LIU Xintao, ZHAO Hui, YAN Wenqing, HE Bipo, LIU Hongyan Chinese Journal of Biological Control    2023, 39 (5): 1156-1171.   DOI: 10.16409/j.cnki.2095-039x.2023.02.055 Abstract （888）      PDF（pc） （1605KB）（30378）       Knowledge map    Save Strain 47M-1 of Penicillium bilaiae showed significant inhibitory effect on a variety of sesame pathogenic fungi. In order to analyze the inhibitiory active substances produced by this strain, liquid chromatography tandem mass spectrometry was used to detect the metabolites of strain cultured for 48 h, 96 h and 144 h. Then, classification of up-regulated differential metabolites in the HMDB database and enrichment of KEGG metabolic pathways were analyzed in order to identify the active metabolites. A total of 105 up-regulated differential metabolites were detected, which were mainly enriched in the superclasses of phenylpropanoids and polyketides. The enrichment analysis of the KEGG metabolic pathways showed that the up-regulated differential metabolites were mainly enriched in metabolic pathways, biosynthesis of cofactors, purine metabolism, biosynthesis of amino acids and ABC transporters. The inhibitiory activities of some up-regulated differential metabolites were verified using medicated plate method. These compounds naringenin, protocatechuic acid, D-(-)-quinic acid, hesperetin, 3-(3-Hydroxyphenyl) propanoic acid, 3, 4-dihydroxyhydrocinnamic acid, 5, 7-dihydroxy-2-(4-hydroxyphenyl)-3, 4-dihydro-2H-1-benzopyran-4-one, rosmarinic acid, bergapten and eriodictyol all showed excellent inhibition activities against sesame pathogenic fungi. In this study, the major groups and key metabolic pathways of potential inhibition metabolites produced by strain 47M-1 were identified, and the inhibitiory activity of some up-regulated differential metabolites was verified. This study will provide data support for the further development and utilization of strain 47M-1 and the research and development of microbial pesticide. Reference | Related Articles | Metrics | Comments（0）

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Mechanism of Chaetomium globosum in Promoting Growth and Preventing Disease and Advance of Its Application and Research FENG Chaohong, LI Lijuan, ZHANG Jiaojiao, WANG Junmei, SONG Yuli, LI Haohai, XU Fei Chinese Journal of Biological Control    2023, 39 (4): 961-969.   DOI: 10.16409/j.cnki.2095-039x.2023.02.043 Abstract （989）      PDF（pc） （432KB）（18248）       Knowledge map    Save Chaetomium globosum, one of common plant endophytic fungi, possesses potential biocontrol function against many plant diseases. This paper summarized the research advances of Chaetomium globosum on biocontrol effect, and its biocontrol mechanisms, which include antibiosis, growth-promoting effect, induction of plant resistance, niche and nutrient competition, and hyperparasitism. Chaetomium globosum exhibits great application potential on plant disease control and growth promotion, soil improvement, and straw degradation. Advances on the fermentation technology and genetic engineering were also illuminated. This study will provide theoretical references for the application research of Chaetomium globosum as biocontrol agent. Reference | Related Articles | Metrics | Comments（0）

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Antifungal Mechanism Analysis and Verification of Bacillus velezensis Ba-0321 Based on Whole Genome Sequencing LI Xiaojie, QIU Rui, LIU Chang, YAO Chenxiao, BAI Jingke, CHEN Yuguo, LI Shujun Chinese Journal of Biological Control    2023, 39 (4): 885-894.   DOI: 10.16409/j.cnki.2095-039x.2023.02.035 Abstract （622）      PDF（pc） （2615KB）（15138）       Knowledge map    Save Bacillus velezensis Ba-0321, isolated from the rhizosphere soil of healthy tobacco plants, is a plant growth promoting rhizobacteria with strong antifungal activity. It has a good potential in biocontrol application. In the study, the inhibitory effects of strain Ba-0321 sterile filtrate on Fusarium oxysporum and Phytophthora nicotianae were determined by methods of co-culture. The whole genome of strain Ba-0321 was sequenced using the second-generation Illumina and the third-generation Nanopore platform, then analyzed for genome assembly, gene functional annotation, prediction of secondary metabolite synthetic gene clusters. The results showed that the sterile filtrate of strain Ba-0321 has inhibitory effects on both F. oxysporum and P. nicotianae. The culture medium containing 10% sterile filtrate has a higher inhibitory rate on the mycelial growth of the two pathogens, reaching 57.38% and 34.30%, respectively. The results of whole genome sequencing showed that the genome size of strain Ba-0321 was 4099109 bp, including 3897 coding genes, and the sequencing data are available in the GenBank database (accession No. CP101904). There were a large number of genes encoding enzymes, terpene, polyketone metabolic pathways, and genes involved in defense mechanisms have been annotated in the GO, COG and KEGG databases. Using anti-SMASH software, 13 secondary metabolite synthesis gene clusters were predicted encoding antibacterial substances such as Surfactin、Fengycin、difficidin、Bacillaene、Bacillibactin、Macrolactin H、Bacilysin. The existence of biosynthetic gene cluster for antibacterial substances and defence machainsm related genes in Ba-0321 strain genome were verified by PCR amplification and sequencing. The enzyme activity test results showed that the strain has resistant enzyme activities such as protease, chitinase, and cellulase.. This study provides a basis for analyzing the antimicrobial mechanism of strain Ba-0321 and mining antifungal related gene resources at the genomic level, which is of a great significance for research and application of the Ba-0321 strain in future. Reference | Related Articles | Metrics | Comments（0）

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Comparative Transcriptomic Profiles Revealed JA- and SA- Regulated Soft Rot Disease Resistance by Bacillus velezensis BCP6 in Amorphophallus konjac ZHANG Meng, CHEN Sen, TANG Dengguo, HUANG Yan, LONG Zhijian, WANG Boya, HU Shanglian, CAO Ying Chinese Journal of Biological Control    2024, 40 (6): 1375-1385.   DOI: 10.16409/j.cnki.2095-039x.2024.02.060 Abstract （469）      PDF（pc） （4362KB）（10486）       Knowledge map    Save The application of Bacillus velezensis has been proven to significantly enhance the resistance to soft rot in Amorphophallus konjac, yet its precise biological control mechanisms remain unclear. This study explored the effects of B. velezensis BCP6 on the defense pathways of A. konjac in response to P. aroidearum MY11 infection through comparative transcriptome analysis. Differential expression analysis, gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) revealed that BCP6 application upregulated pathways related to jasmonic acid (JA), reactive oxygen species, and cell wall synthesis, thereby activating the basic defense system of A. konjac. This immune activation facilitated the rapid initiation of defense responses when subjected to subsequent P. aroidearum infection by enhancing the JA-mediated induced systemic resistance (ISR) and SA-dependent systemic acquired resistance (SAR) signaling pathways. Simultaneously, a significant increase in the expression of genes involved in cell wall formation and pathogenesis-related proteins was observed, effectively enhancing resistance to soft rot. Meanwhile, exogenous JA treatment was found to directly stimulate the expression of cell wall-related genes, namely CESA2 and CSI1, while SA significantly induced the expression of SA-dependent defense genes TLP and E13C. The results provided a theoretical basis for biological control and disease-resistant breeding of konjac. Reference | Related Articles | Metrics | Comments（0）

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Inhibition Effect and Transcriptome Analysis of Isooctyl alcohol on Botrytis cinerea DUAN Tiankun, WANG Yan, YUAN Jiaqi, SU Yaxin, SHI Luxin, WANG Lin, SU Jian, WANG Meiqin, WANG Chunwei Chinese Journal of Biological Control    2023, 39 (6): 1434-1445.   DOI: 10.16409/j.cnki.2095-039x.2023.02.068 Abstract （487）      PDF（pc） （2850KB）（9343）       Knowledge map    Save To reveal the inhibitory mechanism of isooctyl alcohol to Botrytis cinerea, the inhibition effect of isooctyl alcohol on B. cinerea was determined using two-sealed-base-plate method. Mycelial morphology treated with isooctyl alcohol was observed by scanning electron microscopy. The influence of isooctyl alcohol on the extracellular conductivity and the ergosterol synthesis were measured. Response of B. cinerea to isooctyl alcohol was analyzed by RNA sequencing (RNA-Seq). The results showed that the inhibition rate of isooctyl alcohol to mycelia was 67.92%. After treatment with isooctyl alcohol, lots of mycelia were deformed and distorted with the increasing of membrane permeability. At 4, 5 and 6 days after isooctyl alcohol treatment, the ergosterol contents in cell membrane decreased to 44.35%, 38.82% and 42.43%, and the protein concentration decreased to 65.70%, 77.93% and 58.57%, respectively. A total of 577 differentially expressed genes (DEGs), including 312 up-regulated genes and 265 down-regulated genes, were obtained by RNA-Seq. Based on functional analysis of clusters of orthologous groups (COG), gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG), DEGs involved in membrane integral components, ergosterol biosynthesis pathway, amino acid metabolism and ribosome biogenesis pathway were significantly down-regulated, indicating that isooctyl alcohol might affect ergosterol content, energy production and ribosome structure, and further play inhibit role in the growth of B. cinerea. Reference | Related Articles | Metrics | Comments（0）

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Biological Activities of Seven Plant Essential Oils against Panonychus ulmi and GC-MS Analysis of Peppermint Essential Oil Components ZHANG Kun, WANG Kaile, ZHANG Pengjiu, LIU Zhongfang, YANG Jing, FAN Jianbin, ZHAO Jinyu, FAN Renjun, GAO Yue Chinese Journal of Biological Control    2023, 39 (5): 1133-1142.   DOI: 10.16409/j.cnki.2095-039x.2023.01.037 Abstract （486）      PDF（pc） （437KB）（8428）       Knowledge map    Save In order to screen new and green plant-derived acaricides, seven plant essential oils were extracted by steam distillation. The contact killing, fumigation and repellent activities of the essential oils against Panonychus ulmi were determined by Potter spray method, fumigation method and medicine film method. The peppermint essential oil with strong biological activity was screened out, and the chemical components of the peppermint essential oil were identified and analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the LC50 of the peppermint essential oil at 12, 24, and 48 h post spray were 2.13, 0.83, and 0.77 g/L, respectively. The LC50 at 12, 24, and 48 h post fumigation were 382.86, 73.89, and 16.08 μL/L, respectively. The results indicate that the essential oil has strong contact killing and fumigation activities against P. ulmi. Finally, at 24 h post repellent treatment, the peppermint essential oil repelled 77.82% of P. ulmi, which was at grade IV. The peppermint essential oil was analyzed by GC-MS, and a total of 34 chemical components were identified. The detected chemical components accounted for 99.67% of the total essential oil content. The main components were menthol (32.16%), limonene (16.44%), 4-isopropyltoluene (11.32%), 3-carene (10.27%), diethyl malonate (9.57%) and menthone (3.91%). These results show that the peppermint essential oil has strong biological activity against P. ulmi, which provides a scientific basis for the further development of plant-derived acaricides. Reference | Related Articles | Metrics | Comments（0）

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Advances in Biological Control of Tuta absoluta (Meyrick) Based on Predatory Natural Enemy Resources LUO Taotao, YAN Shuyan, CAO Mengyu, ZHANG Jinghang, ZHANG Guifen, WAN Fanghao, ZHANG Ya, LI Tao, LIU Shuangqing, ZHANG Yibo Chinese Journal of Biological Control    2024, 40 (4): 727-738.   DOI: 10.16409/j.cnki.2095-039x.2024.02.039 Abstract （885）      PDF（pc） （432KB）（8037）       Knowledge map    Save Tuta absoluta (Meyrick) is a major invasive alien species worldwide, which mainly damages tomato crops by mining leaves and fruits. Since its invasion in Yili, Xinjiang in 2017, it has spread to many provinces (municipalities and autonomous regions) in China, with outbreaks in some places, seriously threatening the healthy production of China's tomato industry. At present, biological control based on predatory natural enemies is one of the main measures for integrated management of T. absoluta. So far, few review papers have been published on the predatory natural enemy resources of tomato leaf miner. Therefore, this paper systematically sorted out the species of predatory natural enemies of T. absoluta worldwide, summarized the predatory ability of predatory natural enemies of tomato leaf miner, the life parameters of predatory natural enemies of T. absoluta, and the effects of chemical pesticides on the prevention and control of T. absoluta by predatory natural enemies. The existing problems and development direction of T. absoluta control by predatory natural enemies were analyzed in order to provide reference for the biological control of T. absoluta in China. Reference | Related Articles | Metrics | Comments（0）

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Research Progress on Green and Sustainable Management Technologies for Spodoptera frugiperda WEI Lei, NIE Ying, LI Yongjun Chinese Journal of Biological Control    2025, 41 (1): 207-219.   DOI: 10.16409/j.cnki.2095-039x.2024.07.004 Abstract （945）      PDF（pc） （530KB）（7321）       Knowledge map    Save The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is a major worldwide disastrous pest. Since its invasion into China in 2019, the implementation of emergency chemical control measures yielded positive outcomes. However, the occurrence of S. frugiperda in China has now become common and year-round, necessitating a long-term approach for its management. Throughout the history of global pest control, reliance on chemical agents has invariably led to the development of pest resistance. Incidences of pesticide resistance in S. frugiperda have been reported in certain regions of China. Therefore, to mitigate the adverse effects of chemical pesticides and the risk of pesticide resistance, China's management strategies must transition towards green, efficient, precise, and sustainable approaches. On the basis of the regional control strategy and unified management strategy, the Ministry of Agriculture and Rural Affairs has advocated a management framework emphasizing biological control as the focal point, ecological and agricultural control as the foundation, and chemical control as the last resort. This article elaborates on recent technological advancements both domestically and internationally in biological control, ecological and agricultural control methods, with an emphasis on biological control. Additionally, it proposes future research directions and ideas to overcome existing challenges, aiming to provide guidance for the development of green management technologies in China. Reference | Related Articles | Metrics | Comments（0）

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The Behavioral Response of Nilaparvata Lugens to Rice Volatiles Induced by Chilo Suppressalis WANG Xiaolong, SU Shuangli, HU Xiaoyun, YIN Xinming, LI Yunhe Chinese Journal of Biological Control    2023, 39 (4): 970-977.   DOI: 10.16409/j.cnki.2095-039x.2022.11.012 Abstract （510）      PDF（pc） （595KB）（6746）       Knowledge map    Save Previous studies showed that the brown planthoppers (BPH), Nilaparvata lugens, exhibited a strong preference to rice plants that had been infested by the rice striped stem borer (SSB), Chilo suppressalis. In order to identify the rice volatiles that affect the host-searching behavior of BPH, we tested the responses of BPH to 15 key rice volatile compounds induced by SSB infestation using H-tube olfactometer. The results showed that the four compounds including β-caryophyllene, 2-nonanone, germacrene D and (3E)-4,8-dimethyl-1,3,7-nonatriene were significantly attractive to BPH compared to the control (hexane). In contrast, the three compounds including methyl salicylate, 2-undecanone, and (3E,7E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene exhibited significant repellence to BPH. No effect on BPH behaviors were detected in 8 compounds, α-pinene, 2-tridecanone, (E)-2-heptenyl acetate, tridecanal, benzyl benzoate, isophorone, 2-pentadecanone, o-cymene, and isopropyl myristate. In summary, rice plants infested by SSB caterpillar release volatiles both attractive and repellent to BPH, and the preference of BPH to SSB-infested rice plants is caused by combined effects of multiple volatile compounds emitted by SSB-infested plants. These results provide a basis for the preparation of repellents or attractants for controlling the brown planthoppers. Reference | Related Articles | Metrics | Comments（0）

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Research Progress on Damage Characteristics and Parasitoids of Important Pests in Peach SUN Xingxing, WANG Fan, JIANG Yingjie, ZHANG Lisheng Chinese Journal of Biological Control    2025, 41 (1): 231-250.   DOI: 10.16409/j.cnki.2095-039x.2024.03.018 Abstract （359）      PDF（pc） （668KB）（6518）       Knowledge map    Save Prunus persica is one of the vital fruit resources in China, with their growth, development, and fruit yield significantly affected by a wide range of pests. Parasitoid wasps play a crucial role as key biological control agents in effectively managing pests such as peach aphids, peach borer moths, and peach fruit borers. This article provides a comprehensive review of published literature, focusing on the important pests of peach trees and their damage characteristics, the progress in research on related parasitoid wasp resources, and the application of these wasps in integrated pest management. The review elaborated on 21 pests, including mites, that have a significant economic impact on peach trees, and listed 194 species of parasitoid wasps across 20 families that play a pivotal role in the biological control of peach tree pests. Additionally, the article summarized current issues in research on parasitoid wasps for peach tree pests and proposed future research directions, aiming to offer deeper theoretical support for improving pest management strategies and protecting biodiversity in peach orchards. Reference | Related Articles | Metrics | Comments（0）

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Antifungal Activity and Chemical Composition of Five Rosmarinus Essential Oils Controlling Fusarium oxysporum, the Causal Agent of Anoectochilus roxburghii Stem Rot LI Shanshan, ZOU Hui, YANG Min, LINn Jiangbo, DAI Yimin, WU Weijian Chinese Journal of Biological Control    2023, 39 (4): 950-960.   DOI: 10.16409/j.cnki.2095-039x.2023.02.041 Abstract （434）      PDF（pc） （2515KB）（6144）       Knowledge map    Save The present study aims to evaluate the antifungal activitity of 5 Rosmarinus essential oils (EOs) against Fusarium oxysporum, the causal agent of Anoectochilus roxburghii Stem Rot. The phytochemicals of 5 EOs were also determined and qualified by GC-MS methods. Based on the results, The R. officinalis L. 'Severn sea' EO and R. officinalis L. 'Majorca Pink' EO had the strongest activity, the inhibitory rates against F. oxysporum with an EC50 value of 0.15% and 0.25%, more potent than nystatin (0.005%), as a standard antifungal agent. Camphor, eucalyptol and β-pinene were identified as major components in both Rosmarinus officinalis L. 'Severn sea' EO and R. officinalis L. 'Majorca Pink' EO. Reference | Related Articles | Metrics | Comments（0）

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Bionomics and the Control of the Tea Stick Thrips, Dendrothrips minowai Priesner ZHANG Fengge, CAI Xiaoming, LUO Zongxiu, BIAN Lei, LI Zhaoqun, FU Nanxia, ZHOU Li, CHEN Zongmao, XIU Chunli Chinese Journal of Biological Control    2024, 40 (1): 219-228.   DOI: 10.16409/j.cnki.2095-039x.2024.01.001 Abstract （390）      PDF（pc） （1144KB）（5944）       Knowledge map    Save  The tea stick thrips, Dendrothrips minowai Priesner, is one of the most important sucking pests in tea plantations in China. Both D. minowai adults and nymphs feed on fresh and tender leaves, causing leaves to turn curling, brittle and scorched and finallyfall off, which seriously affect the growth of tea plants and the quality of tea products. In this paper, we reviewed the biological and ecological characteristics of D. minowai such as their morphological characteristics, identification method, geographical distribution, damage symptoms, individual development and life cycle, as well as genetic differentiation. In addition, we also summarized the research progress in the management of D. minowai. At the same time, we suggest that the basic research of the olfactory mechanism and the green prevention and control strategy such as monitoring, mass trapping and the use of pathogenic resources in prevention and control of the pest be accelerated. The review provides a theoretical basis and technical support for the integrated pest management of the thrips. Related Articles | Metrics | Comments（0）

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Functional Analysis of ganA in the Nematocidal of Enterobacter ludwigii against Bursaphelenchus xylophilus WANG Haoyu, ZHANG Hongtao, ZHANG Ying, ZHAO Yu, WANG Chuanzhen, ZHANG Lixia, WANG Qi, WU Di, YU Cuifang, LI Haiyong, WANG Shuang, NIU Ben Chinese Journal of Biological Control    2023, 39 (5): 1094-1103.   DOI: 10.16409/j.cnki.2095-039x.2022.11.020 Abstract （383）      PDF（pc） （1355KB）（5670）       Knowledge map    Save Biocontrol microorganisms have been used to control Bursaphelenchus xylophilus disease, but the molecular mechanism of nematicidal is not clear. This study found that the corrected mortality of B. xylophilus treated with E. ludwigii AA4 was more than 85%, and strain AA4 could significantly reduce B. xylophilus infection of pine seedlings. In order to explore the molecular mechanism of AA4 against B. xylophilus, the DNA homologous recombination technique was used to construct the β-galactosidase gene ganA, and the function of ganA in AA4 nematode inhibition was analyzed. The results showed that the B. xylophilus killing activity and B. xylophilus disease prevention in a ganA knockout mutant strain decreased significantly, and the mobility and ability to colonize nematodes of the mutant strain decreased significantly. In addition, compared with the wild-type strain, the ability of the mutant strain to restrict the movement of nematodes was significantly reduced. Therefore, ganA may participate in the process of AA4 killing B. xylophilus by regulating AA4’s ability to colonize B. xylophilus and restrict nematode movement. Reference | Related Articles | Metrics | Comments（0）

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Research Progress on Biological Control of Root Rot Disease in Root- and Rhizome-Derived Traditional Chinese Medicines XIAO Ouli, WANG Jiale, CHEN Jieyin, DAI Xiaofeng, KONG Zhiqiang Chinese Journal of Biological Control    2025, 41 (3): 511-519.   DOI: 10.16409/j.cnki.2095-039x.2025.02.037 Abstract （697）      PDF（pc） （976KB）（5590）       Knowledge map    Save With the scarcity of wild medicinal plant resources and the increase in market demand, conventionally cultivated medicinal plants have become the dominant source in the market. However, due to soil degradation, imbalance in the rhizosphere microbial community, and the accumulation of allelopathic and autotoxic substances, root rot has emerged as a major disease in the cultivation of root- and rhizome-derived traditional Chinese medicines (RR-TCMs) in single, intensive, large-scale cultivation. It has a high incidence rate and is difficult to control, severely affecting the yield and quality of traditional Chinese medicines (TCMs). Currently, the application of microbial biocontrol agents in the control of diseases and pests of medicinal plants offers promising prospects for the sustainable development of TCMs. This paper presents a review of the antagonistic microorganisms and biological control mechanisms of root rot disease in RR-TCMs, aiming to provide a reference for the control of root rot. Reference | Related Articles | Metrics | Comments（0）

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Bioactivity of Four Essential Oils of Artemisia Plants against Drosophila suzukii Matsumura LING Lijun, WANG Yuanyuan, JIANG Kunling, CHENG Wenting, FENG Shenglai, LU Lu, CHEN Xuelin Chinese Journal of Biological Control    2023, 39 (6): 1341-1350.   DOI: 10.16409/j.cnki.2095-039x.2023.01.034 Abstract （534）      PDF（pc） （449KB）（5496）       Knowledge map    Save Drosophila suzukii Matsumura is a serious threat to fruit production around the world due to its fast spread and wide distribution. Essential oils of four Artemisia plants including A. giraldii, A. scoparia, A. anethoides, and A. annua were evaluated for their biological activities against D. suzukii, aiming to exploit new environmentally friendly plant-derived insecticides. The insecticidal activity of the four essential oils against D. suzukii was determined by fumigation test, repellency test and larval mortality test; the chemical composition of the four essential oils was analyzed using gas chromatography-mass spectrometer (GC-MS). The fumigation test showed that the four essential oils all had good insecticidal activities against D. suzukii, and the fumigation activities of A. giraldii essential oil was the best, with LC50 value of 1.421 μL/mL, followed by A. anethoides essential oil, with LC50 value of 3.499 μL/mL, and A. annua essential oil came at the last. The repellent test showed that A. giraldii essential oil was of the highest repellent activities, with repellency indices of 86%, and the A. annua oil was the worst, with repellency indices of 50%. The larval mortality test showed that A. scoparia essential oil resulted in 70% mortality of D. suzukii larvae, while A. anethoides essential oil was the least effective, with only 40% larval mortality. GC-MS analysis showed that there were 82 components in the four essential oils, and β-Caryophyllene, Terpinen-4-ol, and 1,8-Cineole are common compounds in the four essential oils. The above results indicate that the four Artemisia essential oils have the potential to be developed as an insecticide for D. suzukii. This research paves a theoretical foundation for the development of green pollution-free plant-derived pesticides. Reference | Related Articles | Metrics | Comments（0）

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Research Progress and Prospect of DNA Barcoding in Parasitoid Wasps SUN Xingxing, JIANG Yingjie, WANG Fan, ZHANG Lisheng Chinese Journal of Biological Control    2025, 41 (2): 473-491.   DOI: 10.16409/j.cnki.2095-039x.2024.07.007 Abstract （387）      PDF（pc） （530KB）（5470）       Knowledge map    Save Parasitoid wasps play a crucial role in the biological control of agricultural and forestry pests, such as lepidoptera and hemiptera. They have significant potential for development and application in biological control strategies. DNA barcoding, a modern molecular technology widely used in animal and plant biology, ecology, and other fields, has emerged as an important tool for classifying parasitic wasps and conducting ecological research. This paper reviews relevant literature to summarize the primary DNA barcodes and primers utilized for parasitoid wasps. It also discusses the applications of DNA barcoding in taxonomic identification, detection of new or cryptic species, analysis of food web relationships, and phylogenetic research on parasitic wasps. Furthermore, this paper analyzes current challenges faced by DNA barcoding research on parasitic wasps while proposing prospects for key issues such as selecting appropriate DNA barcodes, screening suitable phylogenetic models, and utilizing high-throughput sequencing technology. The aim is to provide theoretical references for resource exploration of parasitic wasps, optimization of biological control strategies, and advancements in evolutionary biology. Reference | Related Articles | Metrics | Comments（0）

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The Nematicidal Activity of Clonostachys pseudochoroleuca Clpse01 on Botrytis cinerea XU Yong, PENG Zijia, XU Zhengmei, SHAO Yakun, LAN Yan, XU Qinghua, YU Zhongdong Chinese Journal of Biological Control    2024, 40 (1): 186-195.   DOI: 10.16409/j.cnki.2095-039x.2023.02.066 Abstract （433）      PDF（pc） （5048KB）（5227）       Knowledge map    Save A parasitic fungus with nematocidal activity, Clpse01, was found while pine wood nematodes (Bursaphelenchus xylophilus) were feeding on Botrytis cinerea media. Clpse01 morphologies and parasite evidences were seen under the microscope. Multiple genes, including ITS, large subunit ribosomal DNA, β-tublin and elongation factor, were used for further limiting by the phylogenic tree. Clpse01's nematocidal effectiveness against pine wood nematodes, as well as its effects on egg hatching and reproduction, were investigated. The Clpse01 fermentation fluid was analyzed for extracellular protein concentrations, β-glucosidase activity, and chitinase activity. Clpse01 was identified as Clonostachys pseudochloleuca using a combination of morphological and molecular phylogenetic approaches. C. pseudochroleuca Clpse01 was discovered entangling and infiltrating B. cinerea hyphae, acquiring nutrients from them while limiting B. cinerea normal growth. Inoculating 2000 pine wood nematodes into media with full growth of isolate Clpse01 resulted in only 6±4 live ones after 8 days, while nematodes isolated from the control medium of B. cinerea reached 33066±6882. The adjusted mortality rate of pine wood nematode treated with C. pseudochroleuca Clpse01 fermentation broth for 48 h was 88.0%, while that treated with 1╳108 conidiospore suspensions per milliliter for 24 h was 88.33%. In double diluent fermentation broth for 24 hours, egg hatching was completely inhibited. After 6 hours of fermentation, the Clpse01 liquid contained 1782.917 μg/mL of total extracellular protein, 9.99 U/mL of β-glucosidase, and 0.8792 U/mL of chitinase. Protein concentrations increased with time. In general, C. pseudochroleuca Clpse01 functions by parasitizing B. cinerea, the feeding fungus, and influencing B. xylophilus reproduction, survival, and egg hatching. Related Articles | Metrics | Comments（0）

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Study on Remediation of Soil Contaminated of Nicosulfuron by Aspergillus terreus DT2 Solid Inoculum ZHANG Jianhua, WANG Xinting, BAI Wenbin, CAO Changlin Chinese Journal of Biological Control    2023, 39 (6): 1463-1473.   DOI: 10.16409/j.cnki.2095-039x.2023.02.056 Abstract （389）      PDF（pc） （485KB）（5131）       Knowledge map    Save In order to solve the problem of nicosulfuron residue in corn field leading to drug injury in the next crop of sorghum, this study prepared DT2 solid bactericide by single factor optimization method, and combined indoor pot experiment with field experiment to add nicosulfuron and apply bactericide DT2 to soil. The repair effect of DT2 solid bactericide on the residual soil of nicosulfuron and its mitigation effect on sorghum were determined. The optimal conditions for solid fermentation of Aspergillus terreus DT2 were pH value 7, inoculation amount 10% and fermentation time 168 h. The effective viable bacteria count can still reach 3.3×108cfu/g after storage at room temperature for 90 days. The results of the indoor pot experiment showed that when the weight of nicosulfuron in soil was 0.023 and 0.046 mg/kg, the inhibition rate of nicosulfuron on sorghum plant height decreased from 26.95% and 49.23% to 1.79% and 2.24% after 30 days of treatment with bacterial agent 6.25 and 12.5 g/kg, respectively. when the weight of nicosulfuron in soil was 0.094 mg/kg, the inhibition rate of nicosulfuron on sorghum plant height was reduced from 51.39% to 4.89% after adding 25 g/kg bactericide for 40 days. When the weight of nicosulfuron in soil was 0.046 mg/kg, adding bacterial agent 12.5 g/kg, 10 days after treatment, the residual rate of nicosulfuron in soil decreased by 56.42% when the weight of nicosulfuron in soil was 0.05 mg/kg, which was significantly different from the control. The mass of nicosulfuron in soil was 0 at 30 days after treatment. DT2 can improve the soil environment, enhance the activity of soil enzymes such as soil urease, alkaline phosphatase, catalase, and soil microbial diversity such as bacteria and fungi. After 40 days of application, nicosulfuron could not only alleviate the inhibition of chlorophyll, net photosynthetic rate and root activity of sorghum, but also promote the growth of sorghum plant height, root length and fresh weight. Field test results showed that the addition of DT2 solid inoculum can effectively reduce the damage of nicosulfuron residue on sorghum, increase the seedling emergence rate and yield, and reduce the disease rate and plant height inhibition rate. When the dosage of nicosulfuron in soil was 20, 30 and 40 g a.i/hm2, adding 300, 450 and 600 kg/hm2 of DT2 solid inoculum, respectively, could reduce the inhibition of nicosulfuron residue on sorghum to the level of no significant toxicity. In conclusion, Aspergillus terreus DT2 bactericide has a good remediation effect on nicosulfuron residues in soil and has potential application prospects. Reference | Related Articles | Metrics | Comments（0）

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Screening and Identification of Biocontrol Potential Myxobacteria Strains against Fire Blight and Pear Canker Diseases and Evaluation of Indoor Control Efficacy BAI Xueying, HAN Jian, SUN Boyuan, SONG Shumin, LUO Ming, SUN Liying Chinese Journal of Biological Control    2023, 39 (6): 1384-1397.   DOI: 10.16409/j.cnki.2095-039x.2023.02.059 Abstract （412）      PDF（pc） （3040KB）（5033）       Knowledge map    Save Fire blight causted by Erwinia amylovora and pear canker causted b Valsa pyri are two major diseases affecting the production of Pyrus sinkiangensis. This study aimed to develop biological control agent for combating fire blight and pear canker diseases by utilizing myxobacteria strains isolated from soil samples collected from different prefectures in Xinjiang. The predation efficiency of myxobacteria strains on E. amylovora was compared by using bacterial lawn co-culture, while the inhibitory effect of myxobacteria strains on hyphal growth and conidial germination of V. pyri was assessed by the pair-culuturing method. Myxobacteria strains with significant predation efficacy and antagonistic activities against E. amylovora and V. pyri were screened, and the control effects of the strains on these two diseases were further evaluted by detached leaves, branches and greenhouse potted experiments. The myxobacteria strains with biocontrol potential were identified based on morphological characteristics, 16S rRNA and lep A gene sequence homology analysis. The results showed that the six selected myxobacteria strains exhibited substantial predation effects on E. amylovora, demonstrated by significant reduction of the bacterial numbers from 109 to 102～105 cfu/mL. Additionally, these strains demonstrated strong antagonistic activity against V. pyri, with inhibition rates of mycelial growth and conidial germination over 70% and 87%, respectively. Strains NST12 and NST47 showed protective and therapeutic effects of over 75% against fire blight on detached P. sinkiangensis leaves and P. betulifolia seedlings, while strains NST47 and NST49 exhibited protective and therapeutic effects of over 72% and 54% against pear canker on detached twigs, respectively. Notably, strain NST47 displayed remarkable biocontrol potential against both fire blight and pear canker. Based on the morphological and molecular identification results, strains NST49 and NST12 were identjfied as Myxococcus fulvus and NST47 was identjfied as Archangium violaceum. Reference | Related Articles | Metrics | Comments（0）

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Isolation and Identification of Arthrobacter pascens S17 and Its Ability to  Promote Growth of Cucumber ZENG Xianfeng, SUN Yuchen, XIE Xuewen, SHI Yanxia, CHAI Ali, LI Baoju, LI Lei Chinese Journal of Biological Control    2024, 40 (1): 178-185.   DOI: 10.16409/j.cnki.2095-039x.2023.02.070 Abstract （317）      PDF（pc） （1772KB）（4919）       Knowledge map    Save  In order to screen out the biocontrol bacteria with growth-promoting effect, soil samples were collected from Hebei, Shandong and other places, and the growth-promoting strains with high-yield IAA ability were isolated and screened, and the potential growth-promoting strains with growth-promoting ability were identified based on morphological characteristics, physiological and biochemical indexes and multi-gene phylogenetic tree analysis, and the growth-promoting effects of growth-promoting strains on cucumber seed germination and cucumber seedlings in pot experiments and field experiments were determined. The results showed that one bacterium, Arthrobacter pascens S17, which had a significant growth-promoting effect on cucumber seedlings, was isolated and identified. Arthrobacter pascens S17 could synthesize IAA in large quantities in DF+ medium, and the highest IAA content was 128.54 μg/mL. The growth rate of cucumber seedling plant height and leaf area of cucumber seedlings reached 35.99% and 26.90% by root irrigation treatment with Arthrobacter pascens S17, and the physiological indexes of cucumber seedlings were significantly higher than those of the control. Arthrobacter pascens S17 has a significant effect on cucumber growth, and has a certain development and application prospect. Related Articles | Metrics | Comments（0）